Sales of BD FACSVerse™ instruments discontinued on July 31st,2019.
Service for BD FACSVerse systems and their related options and upgrades will continue until December 31st, 2023. All current service contracts will be honored. The last date to purchase an annual service contract is January 1st, 2023, for service ending December 31st, 2023.
BD Biosciences may provide service for BD FACSVerse products after December 31st, 2023 based on personnel and parts availability.
Frequently Asked Questions
Q: Will BD Biosciences continue to sell BD FACSVerse spare parts and consumables?
A: After March 31, 2023, our sales of BD FACSVerse spare parts and consumables will be dictated by parts availability and other restrictions.
Q: Will BD Biosciences continue to offer BD FACSVerse operator training?
A: BD FACSVerse training and applications support at the customer’s site will be offered based on availability of staff with product knowledge to provide the training and support. Please contact your regional customer-training center for more information.
Q: How will we obtain service after March 31, 2023?
A: After March 31, 2023, BD FACSVerse instruments may be able to have service performed by BD Biosciences depending on personnel and parts availability.
Reliable Performance for All Users
The compact optical system uses free-space lasers to concentrate intensity at the flow cell. It is designed to minimize light loss and maximize resolution for multicolor applications. A number of innovations built into the optical system—including patented automated laser alignment, smart filter-mirror units for the detector arrays, and a stainless steel flow cell—are designed to maximize reliability and improve system performance.
Smart Heptagon Detector Arrays Ensure Reliability
Compact heptagon detector arrays specially designed for the BD FACSVerse™ system use preassembled filter-mirror units, each with built-in memory chips that precisely identify the spectral information of both the mirror and filter. When the filter-mirror unit is inserted into the heptagon assembly, spectral information is passed from the microprocessor to the cytometer to verify that the unit matches the expected optical configuration. This is another way that the new BD FACSVerse system helps to ensure improved data integrity and ultimately, the reliability of results.
Automated Alignment Adds Consistency
Patented automated alignment and fine alignment features in the BD FACSVerse system also contribute to more consistent performance. Each laser can be independently aligned using a patented 30:1 optical reduction feature to make very small adjustments for achieving optimal alignment.
Better Thermal Stability
Advances in the design of the system’s excitation optics include superior thermal regulation of the lasers. Thermal stability ensures more consistent beam pointing, and as a result, more predictable optical performance.
Smart Components Track Performance
Finally, the optical system in the BD FACSVerse flow cytometer is continuously monitored to help keep it operating at top performance by alerting users to potential maintenance issues before failures occur. The laser and operational status are tracked to help manage configuration with minimal user interaction.
Stainless Steel Flow Cell Adds Stability
The flow cell in the BD FACSVerse analyzer is made of stainless steel and boosts stability in two ways. First, since stainless steel is hydrophilic, its affinity for water helps reduce bubbles for better overall fluidics performance. Second, the lower coefficient of thermal expansion of the stainless steel makes the flow cell less sensitive to temperature fluctuations for more predictable, stable performance.
Best-in-Class Design with Exceptional Flexibility
Through the precise coordination of the optical and fluidics subsystems, the BD FACSVerse™ analyzer offers best-in-class optical detection, sensitivity, and flexibility for multicolor applications.
Vacuum-Based System Enables Flexibility
The BD FACSVerse fluidics system is vacuum-based for exceptional flexibility. This allows users complete freedom to acquire samples manually from virtually any tube format and streamlines workflow. Users simply click the sample tube in place to begin acquisition. The tube holder is built into the cytometer’s chassis to make it more robust and to protect the Sample Injection Tube (SIT) from daily wear.
Sample Input Options to Accommodate Many Needs
The system can accommodate a wide range of input devices in the manual loading position, including 12 x 75-mm tubes made of polystyrene, polypropylene, or K-resin, 15-mL and 50-mL conical tubes, and 1,500-µL or 500-µL microcentrifuge tubes. The optional BD FACS™ Universal Loader further extends this list to include 30- or 40-tube racks with a microplate SBS footprint, deep and standard well 96-well plates, and 384-well plates for maximum flexibility in sample input.
Unique Sample Injection Tube Design for Reliable Results
The unique Sample Injection Tube (SIT) design is key to the fluidics system in the BD FACSVerse analyzer. It consists of a stainless steel outer probe and an inner probe made of a high-performing polymer that descends into the vessel and draws in the sample during acquisition. This allows access to smaller diameter formats without affecting usability. When sample acquisition is complete, the sample injection tubing area is flushed, minimizing carryover. This simplifies workflow and reduces the user’s exposure to aerosols.
Feedback Mechanisms Ensure Stability
An ingenious dual feedback system in the fluidics design controls sheath flow rate to ensure core stream stability. In addition, a thermal feedback loop maintains core stream speed with respect to temperature, giving the BD FACSVerse system unmatched stability over its entire operating temperature range. The BD FACSVerse fluidics system offers two fluidics modes.The standard mode uses three sample flow rates (high, medium, and low) at normal sheath velocity which users can select as required for their application protocols. The high-sensitivity mode uses a lower sheath velocity setting at the medium sample flow rate to support the detection of dimly stained particles. In the high-sensitivity mode, stained cells have a slower transit through the laser interrogation point, which results in higher fluorescence signal resolution.
Optional Volumetric Flow Sensor
The BD Flow sensor option for volumetric measurement measures the volume over the entire acquisition time. It allows the user to reproducibly obtain a precise measure of cells or particles in a volume of sample acquired by the system. It directly determines the volume of the samples as it passes through the sample injection tubing for accurate counts.
Flexible Fluid Supply and Waste Management
For routine use, the 5-L capacity fluidic and waste tanks can be positioned next to the cytometer or within easy access.
Field upgradable extended use tanks with 10-L or 20-L capacity are also available to support up to 8 hours of continuous operation.
Level detection in the fluid tanks tracks the volume of both sheath and waste. Users are alerted when capacity is low, and a shutdown of the system is triggered.
Truly Universal Walkaway Processing
The optional BD FACS™ Universal Loader automates sample handling, enabling rapid processing of multitube racks and microtiter plates in this single unit. Designed to be easy to use and flexible, this option supports walkaway operation for busy research laboratories.
Tube Racks and Plates in the Same Footprint
To ensure simplicity of operation and improve experimental input flexibility, the Loader is designed such that both tube racks and microtiter plates fit into the same compact SBS footprint.
Sample Identification and Imaging Improve Control
The BD FACS Universal Loader software reads barcode labels on tubes and plates when used with a 30-tube rack, matrix-storage tube rack, and all supported microtiter plates. In laboratories that handle high volumes of diverse samples, this helps minimize errors due to mismatches of samples with the assays to be run.
A powerful imaging system in the Loader matches the live image of the sample carrier type loaded into the system with image data stored in the system. This reduces potential errors by verifying that the sample carrier matches what was specified for the user in the worklist, ensuring that tube racks or plates are loaded correctly, and that lids of microtiter plates have been removed. Functional status indicators present on the Loader notify users about loading and unloading status of devices to further support walkaway operation.
Mixing Design for Reliable Sample Resuspension
An orbital shaker built into the Loader vortexes samples in tubes or microtiter plates. This design maximizes flexibility so that users can load prepared samples onto the Loader without needing to manually resuspend them. A selection of mixing frequencies, optimized for multiple types of cells, ensures adequate mixing while preserving the cellular viability of the sample.
Universal Experience, Ergonomic Design
The BD FACSVerse system was designed to ensure that the customer experience was equivalent with or without the BD FACS Universal Loader option. To maintain the system flexibility, the manual station is always accessible, even when the Loader is present. The Loader is designed to keep the footprint as compact as possible and is ergonomically situated so that the system workstation is to the right. The Loader’s indicator lights and warning systems have been designed to ensure that a user seated at the workstation can easily see the Loader status.
Taking Productivity to the Next Level
A true evolution in flow cytometry is empowered by BD FACSuite™ software. It enables the BD FACVerse™ analyzer to provide the ultimate in ease of use and flexibility while generating the highest quality reproducible data possible with the technology available today.
BD FACSuite software minimizes the number of user interactions with the instrument so that the user can spend more time working with samples. BD FACSuite software users can complete tasks at the click of a button for key workflows, from startup through instrument setup, sample acquisition, data analysis, and system shutdown.
BD FACSuite software streamlines instrument setup to achieve a new level of ease of use and reproducibility in cytometer setup and subsequent analysis.
BD FACSuite™ CS&T Research Beads are used to check the cytometer performance and automatically make adjustments, ensuring consistent values from day to day and experiment to experiment. Comprehensive checks during system initialization and setup, including performance QC, verify system performance, ensures that detector settings are optimal and triggers the automated laser alignment process if required. The entire setup process can be performed without manual intervention.
A New Approach Enabling Easy, Flexible Compensation
Accurate compensation is absolutely necessary for proper analysis of multicolor flow cytometric experiments, but it has always been a challenging and time-consuming process for users.
In the past, generating accurate compensation matrices typically required running individual compensation controls for every fluorochrome used at least once a day. Thus, for a five-color experiment, all five fluorochromes would be run initially to determine their spillover values (SOVs) for a compensation matrix. To subsequently add a sixth fluorochrome to the experiment, users would have to re-run all six at the same time.
BD FACSuite software minimizes the frequency and number of compensation controls required by taking advantage of the accurate, reproducible instrument setup provided by a combination of BD FACSuite™ CS&T Research Beads and BD FACSuite™ FC Beads. A single 20-minute procedure using BD FACSuite FC Beads determines SOVs that are gain-independent. These values are valid for 30 days. To add a new fluorochrome to the system, users run just one control for the new fluorochrome. Finally, the calculation of gain-independent SOVs lets users modify PMT voltages if required without re-running compensation controls. All SOVs are automatically recalculated.
Together, these features mean time savings every day, since the BD FACSVerse system significantly reduces the frequency and complexity of the compensation process. It does this while increasing the accuracy and reproducibility of SOVs, and therefore the final experimental results.
Automatic Startup, System Idle, and Shutdown
To increase laboratory efficiency and ensure the system is ready by a specified time each day, a timer in the software can be set to turn on the instrument and complete initialization and warmup routines. BD FACSuite software can also be programmed to shut down the BD FACSVerse system at the end of the worklist run or when the system is idle for a specified period of time. In the case of idle time shutdown, a dialog communicates that the system will be shutting down, so that users can cancel the shutdown if desired.
Streamlined Approach to Application Support
BD FACSuite™ software provides optional BD pre-defined research assays for apoptosis, cell cycle, cell proliferation, and cytokine detection that are matched with BD reagent kits for reproducible results.
User-defined experiments created in BD FACSuite software can be converted to reusable assays that include relevant settings, acquisition and analysis worksheets, gating strategies, and report parameters to reduce variability of data between users and between laboratories for the same application.
In addition, before samples are acquired, the software verifies that the system’s current configuration matches the configuration needed to run the experiment. If it identifies a mismatch, such as a different optical filter-mirror unit, the system alerts the user. This check can help streamline workflow and reliability of results.
Intuitive Workspaces for Ease of Adoption
The user interface is designed around three common workflows: setup, acquisition, and analysis. Workspaces provide users the functionality they need to perform the tasks relevant to a given workflow. For example, in the Setup workspace, users find the QC tools and tools for instrument configuration. In the Experiment workspace, users can adjust tube settings, plots and gates. These workflow-based workspaces reduce clutter to help users complete their tasks more quickly. Administrative functions such as user management, diagnostics and periodic maintenance procedures are accessible only to authorized personnel.
Multitasking Support for Added Productivity
The functions in BD FACSuite software workspaces operate both together and independently. The software supports multitasking, enabling users to work on several tasks simultaneously, such as performing data analysis while the system is acquiring data. A user even can prioritize a single sample for immediate analysis. Multitasking is yet another way that the software promotes a more flexible, productive workflow.
Reliable Data Across Sites
For the first time, researchers have control to ensure consistent instrument setup and consistent experiment results (acquisition, analysis, gating strategies, and report parameters) in a single application—BD FACSuite software. Free from concerns about instrument and human variables, scientists can move forward in their research, reliably reproducing an experiment from one instrument to another and across sites.
Support for Security Standards and More
BD FACSuite software supports 21 CFR Part 11 guidelines for Electronic Records and Signatures to protect data integrity. This includes unique user identification and passwords, electronic signatures, and audit trails. In addition to meeting the requirements specified in the 21 CFR Part 11 guidelines, BD FACSuite software encrypts files and uses specially designed algorithms to ensure that data is not manipulated by unauthorized entities.
Optional Remote Systems Management
Optional remote systems management software enables BD field service personnel to access a networked BD FACSVerse™ system and review system performance and status using the System Health Report. This allows the BD service team to determine the nature and extent of the issue, and if possible repair the issue remotely to ensure minimal downtime. It also helps the BD service team to be better prepared if on-site support is required.
Greater Speed and Freedom for Data Analysis
BD FACSuite software offers a variety of ways to visualize data and tools to easily look more closely at data for analysis.
The data analysis tools let users examine data as dot plots, histograms, contours and density plots. For a better look at data, new hybrid contour plots combine standard dot plots with the coloring of contour plots. The information about contour statistics is available by hovering over the plot. A new capability lets users overlay dot plots within the same or different files. Gating tools include ellipses, polygons and free-hand polygons, as well as the traditional rectangle. Gates can be rotated as needed, and with a zoom feature, users can zoom in or out of areas of interest.
Several types of new quadrant markers provide additional flexibility, including a hinged marker to more precisely identify quadrants of interest.
An expression editor is also built into BD FACSuite software to speed analysis. The expression editor lets users develop mathematical expressions and assign them to keywords. Users can hover over a result for a display of the formula that was used to calculate it.
To assist with presentation, vector graphics provide superior quality images that can be easily cut and pasted into third-party software such as Microsoft® PowerPoint®. A new report generator produces high-quality reports that include color graphics and can be easily customized by dragging and dropping report elements.
- BD FACSuite™ Research Assays: Cell Cycle Research
- BD FACSuite™ Research Assays: Apoptosis Research
- BD FACSuite™ Research Assays: Immune Function Research
- Cell Cycle and DNA Content Analysis Using the BD Cycletest™ Assay on the BD FACSVerse™ System
BD FACSVerse™ Violet Laser (405 nm)
- BD Horizon™ BV421 (ex 407 nm/em 421 nm)
- BD Horizon™ V450 (ex 404 nm/em 448 nm)
- Pacific Blue™ (ex 401 nm/em 452 nm)
- AmCyan (ex 457 nm/em 491 nm)
- BD Horizon™ V500 (ex 415 nm/em 500 nm)
- BD Horizon™ BV510 (ex 405 nm/em 510 nm)
- BD Horizon™ BV605 (ex 407 nm/em 602 nm)
BD FACSVerse Blue Laser (488 nm)
- FITC (ex 494 nm/em 519 nm)
- Alexa Fluor® 488 (ex 495 nm/em 519 nm)
- PE (ex 496 nm/em 578 nm)
- PerCP (ex 482 nm/em 678 nm)
- PerCP-Cy™5.5 (ex 482 nm/em 695 nm)
- PE-Cy™7 (ex 496 nm/em 785 nm)
BD FACSVerse Red Laser (640 nm)
- Assay Portability on the BD FACSVerse™ System
- Cell Cycle and DNA Content Analysis Using the BD Cycletest Assay on the BD FACSVerse™ System
- Cell Cycle Analysis Using the BD BrdU FITC Assay on the BD FACSVerse™ System
- Detection of Apoptosis Using the BD Annexin V FITC Assay on the BD FACSVerse™ System
- Detection of Intracellular Cytokines in T Lymphocytes using the BD FastImmune™ Assay on the BD FACSVerse™ System
- Quantification of Cytokines Using BD™ Cytometric Bead Array on the BD™ FACSVerse System and Analysis in FCAP Array™ Software
- Studying Mouse Thymocyte Development using Multiparametric Flow Cytometry: An Efficient Method to Improve an 8-Color Panel on the BD FACSVerse™ System
Product Information Sheets
- BD Assurity Linc™
- BD Assurity Linc™ Site Survey Form
- BD FACSDiva™ Software Standardizing Application Settings Across Multiple Cytometers
- BD FACSuite™ CS&T Research Beads
- BD FACSuite™ Data Security
- BD FACSuite™ Research Assays: Apoptosis Research
- BD FACSuite™ Research Assays: Cell Cycle Research
- BD FACSuite™ Research Assays: Immune Function Research
- BD FACSuite™ Software: Standardizing Application Settings Across Multiple Cytometers
- BD Flow Cytometry Reagents: Multicolor Panels Designed for Optimal Resolution with the BD FACSVerse™ Flow Cytometer
Quick Reference Guides
- Cross-Instrument and Cross-Site Standardization Using BD FACSDiva Software's Custom Application Settings: Part I of II
- Cross-Instrument and Cross-Site Standardization Using BD FACSDiva Software's Custom Application Settings: Part II of II
- Instrument Characterization and Performance Tracking for Digital Flow Cytometers
- The Digital Flow Cytometer: Performing Instrument Characterization for Optimal Setup
- Using the BD™ Cytometer Setup and Tracking (CS&T) System for Instrument Characterization and Performance Tracking
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Class 1 Laser Product.
Alexa Fluor® is a registered trademark and Pacific Blue™ is a trademark of Molecular Probes, Inc.
Cy™ is a trademark of Amersham Biosciences Corp. Cy™ dyes are subject to proprietary rights of Amersham Biosciences Corp and Carnegie Mellon University and are made and sold under license from Amersham Biosciences Corp only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from Amersham Biosciences Corp, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.