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RY586 Rat Anti-Mouse CD100 (Sema4D)
Product Details
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BD OptiBuild™
Semaphorin-4D; SEM4D; Semaphorin-C-like 2; Semacl2; Semaj; M-Sema G; coll-4
Mouse (Tested in Development)
Rat IgG2a, λ
Mouse CD100 Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
20354
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Researchers should determine the optimal concentration of this reagent for their individual applications.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. CF™ is a trademark of Biotium, Inc.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
753293 Rev. 1
Antibody Details
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BMA-12

The BMA-12 monoclonal antibody specifically binds to CD100 which is also known as, Semaphorin-4D (Sema4D), or Collapsin-4 (Coll-4). CD100 is a ~150 kDa type I transmembrane glycoprotein that belongs to the class IV Semaphorin subfamily within the Ig gene superfamily. CD100 is expressed by cells from a broad range of tissues including the nervous system, where it serves in axon guidance and synapse formation, and the kidney. It is expressed by most hematopoietic cells including T cells which express CD100 more abundantly than B cells and dendritic cell subsets. Its expression is significantly upregulated upon activation of these cell types. CD100 can costimulate T cell responses. For B cells and dendritic cells, CD100 interaction with its ligands, CD72 or Plexin B1, induces cellular activation and promotes survival, respectively. CD100 is also known to interact with Plexin B2 on skin gamma delta T cells and contribute to the repair of tissue damage caused by inflammation. A biologically-active, soluble form of CD100 is generated by proteolytic cleavage of the cell surface form.

753293 Rev. 1
Format Details
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RY586
The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
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RY586
Yellow-Green 561 nm
564 nm
586 nm
753293 Rev.1
Citations & References
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View product citations for antibody "753293" on CiteAb

Development References (6)

  1. Kikutani H, Kumanogoh A. Semaphorins in interactions between T cells and antigen-presenting cells. Nat Rev Immunol. 2003; 3(2):159-167. (Biology). View Reference
  2. Kumanogoh A, Watanabe C, Lee I, et al. Identification of CD72 as a lymphocyte receptor for the class IV semaphorin CD100: a novel mechanism for regulating B cell signaling. Immunity. 2000; 13(5):621-631. (Immunogen: Blocking, ELISA, Flow cytometry). View Reference
  3. Li DH, Tung JW, Tarner IH, et al. CD72 down-modulates BCR-induced signal transduction and diminishes survival in primary mature B lymphocytes. J Immunol. 2006; 176(9):5321-5328. (Biology). View Reference
  4. Meehan TF, Witherden DA, Kim CH, et al. Protection against colitis by CD100-dependent modulation of intraepithelial gammadelta T lymphocyte function. Mucosal Immunol. 2014; 7(1):134-142. (Biology). View Reference
  5. Smith EP, Shanks K, Lipsky MM, DeTolla LJ, Keegan AD, Chapoval SP. Expression of neuroimmune semaphorins 4A and 4D and their receptors in the lung is enhanced by allergen and vascular endothelial growth factor.. BMC Immunol. 2011; 12:30. (Clone-specific: Flow cytometry). View Reference
  6. Wang X, Kumanogoh A, Watanabe C, Shi W, Yoshida K, Kikutani H. Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune responses. Blood. 2001; 97(11):3498-3504. (Clone-specific: ELISA, Flow cytometry, Immunoaffinity chromatography, Immunoprecipitation). View Reference
View All (6) View Less
753293 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.