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RY586 Mouse Anti-Human IL-9 Receptor Alpha (CD129)
Product Details
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BD OptiBuild™
CD129; Interleukin-9 receptor; IL-9R alpha chain; IL-9Rα
Human (Tested in Development)
Mouse IgG2b, κ
Human IL9R Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Researchers should determine the optimal concentration of this reagent for their individual applications.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. CF™ is a trademark of Biotium, Inc.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  10. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
753383 Rev. 1
Antibody Details
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AH9R7

The AH9R7 monoclonal antibody specifically binds to the Interleukin-9 Receptor (IL-9R, IL-9Rα) also known as CD129. CD129 is a Type I transmembrane glycoprotein and a Type I Cytokine Receptor (Hemopoietin) Superfamily member. The high-affinity, signaling IL-9 Receptor complex is comprised of CD129 (IL-9 Receptor alpha chain subunit) and CD132 (common γ chain/γc).  CD129 is expressed by hematopoietic progenitors, thymocytes, T cells, B cells, macrophages, eosinophils, mast cells, epithelia cells, muscle cells and neurons.  IL-9 signals through the IL-9R complex (ie, through JAK, STAT and related signal pathways) to regulate the growth, proliferation, differentiation and survival of myeloid and erythroid progenitor cells, thymocytes, T cells, B cells and some tumor cells.

753383 Rev. 1
Format Details
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RY586
The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
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RY586
Yellow-Green 561 nm
564 nm
586 nm
753383 Rev.1
Citations & References
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View product citations for antibody "753383" on CiteAb

Development References (6)

  1. De Smedt M, Verhasselt B, Kerre T, et al. Signals from the IL-9 receptor are critical for the early stages of human intrathymic T cell development. J Immunol. 2000; 164(4):1761-1767. (Clone-specific: Inhibition). View Reference
  2. Demoulin JB, Uyttenhove C, Van Roost E, et al. A single tyrosine of the interleukin-9 (IL-9) receptor is required for STAT activation, antiapoptotic activity, and growth regulation by IL-9. Mol Cell Biol. 1996; 16(9):4710-4716. (Clone-specific: Flow cytometry, Immunoprecipitation). View Reference
  3. Grasso L, Huang M, Sullivan CD, et al. Molecular analysis of human interleukin-9 receptor transcripts in peripheral blood mononuclear cells. Identification of a splice variant encoding for a nonfunctional cell surface receptor. J Biol Chem. 1998; 273(37):24016-24024. (Clone-specific: Flow cytometry, IC/FCM Block, Immunohistochemistry, Immunoprecipitation). View Reference
  4. Pilette C, Ouadrhiri Y, Van Snick J, et al. IL-9 inhibits oxidative burst and TNF-alpha release in lipopolysaccharide-stimulated human monocytes through TGF-beta. J Immunol. 2002; 168(8):4103-4111. (Clone-specific: Flow cytometry, Inhibition). View Reference
  5. Pilette C, Ouadrhiri Y, Van Snick J, et al. Oxidative burst in lipopolysaccharide-activated human alveolar macrophages is inhibited by interleukin-9. Eur Respir J. 2002; 20:1198-1205. (Clone-specific: Functional assay, Inhibition). View Reference
  6. Wilhelm C, Turner JE, Van Snick J, Stockinger B. The many lives of IL-9: a question of survival?. Nat Immunol. 2012; 13(7):637-641. (Biology). View Reference
View All (6) View Less
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.