The site is undergoing maintenance on
If you encounter problems while ordering, please contact us at BDBCustomerService@bd.com
See More
Product Details
BD Pharmingen™
Transferrin Receptor; TR; TfR; TfR1; Tfrc; Trfr; Mtvr-1
Mouse (QC Testing)
Rat WF, also known as Wistar Furth IgG1, κ
Mouse cell line
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunofluorescence, Inhibition (Reported), Immunohistochemistry-paraffin (Not Recommended)
0.5 mg/ml
AB_394742
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures
IHC of paraffin-embedded sections is not recommended. Other reported applications include in vitro inhibition of cell proliferation and immunofluorescence microscopy.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Purified Rat IgG1, κ Isotype Control RUO
Size
0.5 mg
Cat No.
553922
PE Rat Anti-Mouse TER-119/Erythroid Cells RUO
Size
0.2 mg
Cat No.
553673
FITC Mouse Anti-Rat IgG1 RUO
Size
0.5 mg
Cat No.
553892
553264 Rev. 13
Antibody Details
C2
The C2 monoclonal antibody specifically binds to CD71, the transferrin receptor. CD71 is a disulfide-linked homodimer of 95-kDa subunits. CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell's iron requirements. It is expressed at high levels on developing erythroid cells, and it is upregulated after mitogenic activation of B or T lymphocytes. The C2 monoclonal antibody selectivity inhibits some types of T- and B-cell activation by down-regulation of transferrin receptor expression, but it does not block binding of transferrin.
553264 Rev. 13
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553264 Rev.13
Citations & References
View product citations for antibody "553264" on CiteAb
Development References (5)
-
Fujimoto T. GPI-anchored proteins, glycosphingolipids, and sphingomyelin are sequestered to caveolae only after crosslinking. J Histochem Cytochem. 1996; 44(8):929-941. (Clone-specific: Immunofluorescence). View Reference
-
Kemp JD, Thorson JA, Gomez F, Smith KM, Cowdery JS, Ballas ZK. Inhibition of lymphocyte activation with anti-transferrin receptor Mabs: a comparison of three reagents and further studies of their range of effects and mechanism of action. Cell Immunol. 1989; 122(1):218-230. (Clone-specific: Activation, Inhibition). View Reference
-
Kemp JD, Thorson JA, McAlmont TH, Horowitz M, Cowdery JS, Ballas ZK. Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols. J Immunol. 1987; 138(8):2422-2426. (Immunogen: Activation, Inhibition). View Reference
-
Lok CN, Loh TT. Regulation of transferrin function and expression: review and update. Biol Signals Recept. 1998; 7(3):157-178. (Biology). View Reference
-
Thorson JA, Smith KM, Gomez F, Naumann PW, Kemp JD. Role of iron in T cell activation: TH1 clones differ from TH2 clones in their sensitivity to inhibition of DNA synthesis caused by IgG Mabs against the transferrin receptor and the iron chelator deferoxamine. Cell Immunol. 1991; 134(1):126-137. (Clone-specific: Activation, Inhibition). View Reference
553264 Rev. 13
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.