Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Brazil (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified NA/LE Rat Anti-Mouse CD49e
      Alert icon
      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Itga5; ITA5; Integrin alpha-5; Fibronectin receptor alpha; Fnra; VLA5 alpha
      Mouse (QC Testing)
      Rat LEW, also known as Lewis IgG2a, κ
      Mouse Mast Cell Line MC/9
      Flow cytometry (Routinely Tested), (Co)-stimulation, Blocking, Immunofluorescence, Immunohistochemistry-frozen (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
      1.0 mg/ml
      AB_394778
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      553318 Rev. 12
      Antibody Details
      Down Arrow Up Arrow
      5H10-27 (MFR5)

      The 5H10-27 (MFR5) monoclonal antibody specifically binds to the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, precursor and activated B cells, mast cells, platelets, epithelial and endothelial cells, fibroblasts and a variety of mouse cell lines. Soluble 5H10-27 (MFR5) antibody has been reported to inhibit VLA-5-mediated functions in vitro. In addition, immobilized 5H10-27 (MFR5) antibody reportedly costimulates the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody.

      553318 Rev. 12
      Format Details
      Down Arrow Up Arrow
      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      553318 Rev.12
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "553318" on CiteAb

      Development References (10)

      1. Cukierman E, Pankov R, Stevens DR, Yamada KM. Taking cell-matrix adhesions to the third dimension. Science. 2001; 294(5547):1708-1712. (Clone-specific: Immunofluorescence, Immunohistochemistry). View Reference
      2. Gomez M, Cano A. Expression of beta 1 integrin receptors in transformed mouse epidermal keratinocytes: upregulation of alpha 5 beta 1 in spindle carcinoma cells. Mol Carcinog. 1995; 12(3):153-165. (Clone-specific: Immunofluorescence, Immunohistochemistry). View Reference
      3. Halvorson MJ, Coligan JE. Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes. J Immunol. 1995; 155(10):4567-4574. (Clone-specific: Blocking). View Reference
      4. Hardy CL, Minguell JJ. Modulation of the adhesion of hemopoietic progenitor cells to the RGD site of fibronectin by interleukin 3. J Cell Physiol. 1995; 164(2):315-323. (Biology). View Reference
      5. Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994; 20(1):25-44. (Immunogen: Blocking). View Reference
      6. Rich S, Van Nood N, Lee HM. Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. J Immunol. 1996; 157(7):2916-2923. (Clone-specific: Blocking, (Co)-stimulation). View Reference
      7. Ruppert M, Aigner S, Hubbe M, Yagita H, Altevogt P. The L1 adhesion molecule is a cellular ligand for VLA-5. J Cell Biol. 1995; 131(6):1881-1891. (Clone-specific: Blocking). View Reference
      8. Schultz JF, Armant DR. Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. Regulation by ligand-induced mobilization of stored receptor. J Biol Chem. 1995; 270(19):11522-11531. (Clone-specific: Blocking). View Reference
      9. Uhlenkott CE, Huijzer JC, Cardeiro DJ, Elstad CA, Meadows GG. Attachment, invasion, chemotaxis, and proteinase expression of B16-BL6 melanoma cells exhibiting a low metastatic phenotype after exposure to dietary restriction of tyrosine and phenylalanine. Clin Exp Metastasis. 1996; 14(2):125-137. (Clone-specific: Blocking). View Reference
      10. Yang JT, Hynes RO. Fibronectin receptor functions in embryonic cells deficient in alpha 5 beta 1 integrin can be replaced by alpha V integrins. Mol Biol Cell. 1996; 7(11):1737-1748. (Clone-specific: Immunofluorescence, Immunohistochemistry). View Reference
      View All (10) View Less
      553318 Rev. 12

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.