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      PE Rat Anti-Mouse CD71
      PE Rat Anti-Mouse CD71
      Two-color analysis of the expression of CD71 on developing erythroid cells. BALB/c bone-marrow leukocytes were simultaneously stained with biotinylated anti-mouse Erythroid Cells mAb TER-119 (Cat. no. 553672) and PE-conjugated mAb C2 (Right panel), followed by Avidin-FITC (Cat. no. 554057). Flow cytometry was  performed on a BD FACScan™ flow cytometry system.
      PE Rat Anti-Mouse CD71
      Two-color analysis of the expression of CD71 on developing erythroid cells. BALB/c bone-marrow leukocytes were simultaneously stained with biotinylated anti-mouse Erythroid Cells mAb TER-119 (Cat. no. 553672) and PE-conjugated mAb C2 (Right panel), followed by Avidin-FITC (Cat. no. 554057). Flow cytometry was  performed on a BD FACScan™ flow cytometry system.
      Product Details
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      BD Pharmingen™
      Transferrin Receptor; TR; TfR; TfR1; Tfrc; Trfr; Mtvr-1
      Mouse (QC Testing)
      Rat WF, also known as Wistar Furth IgG1, κ
      Mouse cell line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      22042
      AB_394744
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      This antibody conjugate is compatible with intracellular staining protocols using the BD Cytofix/Cytoperm™ Kit (Cat. no 554714).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      561937 Rev. 2
      Antibody Details
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      C2

      The C2 monoclonal antibody specifically binds to CD71, the transferrin receptor. CD71 is a disulfide-linked homodimer of 95-kDa subunits. CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell's iron requirements. It is expressed at high levels on developing erythroid cells, and it is upregulated after mitogenic activation of B or T lymphocytes. The C2 monoclonal antibody selectivity inhibits some types of T- and B-cell activation by down-regulation of transferrin receptor expression, but it does not block binding of transferrin.

      561937 Rev. 2
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      561937 Rev.2
      Citations & References
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      View product citations for antibody "561937" on CiteAb

      Development References (4)

      1. Kemp JD, Thorson JA, Gomez F, Smith KM, Cowdery JS, Ballas ZK. Inhibition of lymphocyte activation with anti-transferrin receptor Mabs: a comparison of three reagents and further studies of their range of effects and mechanism of action. Cell Immunol. 1989; 122(1):218-230. (Clone-specific: Activation). View Reference
      2. Kemp JD, Thorson JA, McAlmont TH, Horowitz M, Cowdery JS, Ballas ZK. Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols. J Immunol. 1987; 138(8):2422-2426. (Immunogen: Activation). View Reference
      3. Lok CN, Loh TT. Regulation of transferrin function and expression: review and update. Biol Signals Recept. 1998; 7(3):157-178. (Biology). View Reference
      4. Thorson JA, Smith KM, Gomez F, Naumann PW, Kemp JD. Role of iron in T cell activation: TH1 clones differ from TH2 clones in their sensitivity to inhibition of DNA synthesis caused by IgG Mabs against the transferrin receptor and the iron chelator deferoxamine. Cell Immunol. 1991; 134(1):126-137. (Clone-specific: Activation). View Reference
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      561937 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.