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BD Pharmingen™ PE Mouse Anti-Human Transferrin R (CD71)
Clone OKT9 (also known as OKT-9; OKT 9) (RUO)
Flow cytometric analysis of Transferrin R (CD71) expression on activated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated for 3 days with Phytohemagglutinin (PHA). The cells were harvested, preincubated with Human BD Fc Block™ (Cat. No. 564219/564220), and then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Transferrin R (CD71) antibody (Cat. No. 566722/566723; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing CD71 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) activated human peripheral mononuclear cells. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Flow Cytometer System and FlowJo™ software.
Flow cytometric analysis of Transferrin R (CD71) expression on activated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated for 3 days with Phytohemagglutinin (PHA). The cells were harvested, preincubated with Human BD Fc Block™ (Cat. No. 564219/564220), and then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Transferrin R (CD71) antibody (Cat. No. 566722/566723; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing CD71 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) activated human peripheral mononuclear cells. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Flow Cytometer System and FlowJo™ software.
Product Details
BD Pharmingen™
TfR; sTfR; TFRC; Trfr; TfR1; TR; T9; p90
Human (QC Testing)
Mouse BALB/c x A/J, also known as CAF1 IgG1, κ
Human T-cell acute lymphoblastic leukemia cells
Flow cytometry (Routinely Tested)
5 µl
AB_2744492
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Human BD Fc Block™ RUO
Size
50 µg
Cat No.
564219
Human BD Fc Block™ RUO
Size
0.25 µg
Cat No.
564220
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
Cell Viability Solution RUO
Size
500 Tests
Cat No.
555815
566723 Rev. 1
Antibody Details
OKT9
The OKT9 monoclonal antibody specifically binds to CD71 which is also known as the transferrin receptor (TFR or Transferrin R). CD71 (Transferrin R) is a type II transmembrane glycoprotein that is encoded by TFRC (transferrin receptor) which belongs to the Transferrin receptor family. It is expressed on cells as a disulfide-linked homodimer comprised of ~95 kDa monomers. CD71 is expressed on early thymocytes as well as activated lymphocytes, monocytes, macrophages, brain endothelium, and most proliferating cells. The transferrin receptor is also present on early erythroid cells but is lost as reticulocytes differentiate into mature erythrocytes. CD71 is lowly expressed on normal resting lymphocytes and is upregulated during lymphocyte responses to antigens or mitogens. The transferrin receptor is essential for iron transport into proliferating cells. Through an endocytic pathway, the transferrin receptor mediates cellular iron uptake by binding and internalizing iron that is bound to transferrin. After releasing iron within the low pH endosomal environment, transferrin and its receptor can be recycled to the cell surface.
566723 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
566723 Rev.1
Citations & References
View product citations for antibody "566723" on CiteAb
Development References (4)
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Goding JW, Burns GF. Monoclonal antibody OKT-9 recognizes the receptor for transferrin on human acute lymphocytic leukemia cells.. J Immunol. 1981; 127(3):1256-8. (Clone-specific: Immunoprecipitation). View Reference
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Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980; 77(3):1588-1592. (Immunogen: Flow cytometry). View Reference
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Schwarting R, Stein H. Cluster report: CD71. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:455-460.
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Zola H, Swart B, Nicholson I, Voss E. CD71. In: Zola H. Leukocyte and Stromal Cell Molecules : the CD Markers. Hoboken, N.J.: Wiley-Liss; 2007:163.
566723 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.