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TGFb_PE_TDS-2-1.png

Flow cytometric analysis of human TGF-β1 expressed by TGF-β1-transfected P3UI cells. Left Panel: Untransfected mouse P3UI myeloma cells (shaded histogram) and human TGF-β1-transfected P3UI cells (open histogram) were surface stained with PE Mouse anti-Human TGF-β1 antibody (Cat. No. 562339) using BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656). Right Panel: Untransfected (shaded histogram) and Human TGF- β1 transfected (open histogram) P3UI cells were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722), washed with BD Perm/Wash™ Perm/Wash Buffer (Cat. No. 554723), and then stained with PE Mouse Anti-Human TGF- β1 (Cat. No. 562339). Flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

562339Image1.png

Flow cytometric analysis of human TGF-β1 expressed by TGF-β1-transfected P3UI cells. Left Panel: Untransfected mouse P3UI myeloma cells (shaded histogram) and human TGF-β1-transfected P3UI cells (open histogram) were surface stained with PE Mouse anti-Human TGF-β1 antibody (Cat. No. 562339) using BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656). Right Panel: Untransfected (shaded histogram) and Human TGF- β1 transfected (open histogram) P3UI cells were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722), washed with BD Perm/Wash™ Perm/Wash Buffer (Cat. No. 554723), and then stained with PE Mouse Anti-Human TGF- β1 (Cat. No. 562339). Flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

TGFb_PE_TDS-2-1.png 562339Image1.png Red-Cap-Blue-Label.jpg

Flow cytometric analysis of human TGF-β1 expressed by TGF-β1-transfected P3UI cells. Left Panel: Untransfected mouse P3UI myeloma cells (shaded histogram) and human TGF-β1-transfected P3UI cells (open histogram) were surface stained with PE Mouse anti-Human TGF-β1 antibody (Cat. No. 562339) using BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656). Right Panel: Untransfected (shaded histogram) and Human TGF- β1 transfected (open histogram) P3UI cells were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722), washed with BD Perm/Wash™ Perm/Wash Buffer (Cat. No. 554723), and then stained with PE Mouse Anti-Human TGF- β1 (Cat. No. 562339). Flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of human TGF-β1 expressed by TGF-β1-transfected P3UI cells. Left Panel: Untransfected mouse P3UI myeloma cells (shaded histogram) and human TGF-β1-transfected P3UI cells (open histogram) were surface stained with PE Mouse anti-Human TGF-β1 antibody (Cat. No. 562339) using BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656). Right Panel: Untransfected (shaded histogram) and Human TGF- β1 transfected (open histogram) P3UI cells were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722), washed with BD Perm/Wash™ Perm/Wash Buffer (Cat. No. 554723), and then stained with PE Mouse Anti-Human TGF- β1 (Cat. No. 562339). Flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Pharmingen™
TGFB1; TGFbeta1; TGF-beta-1; Transforming growth factor, beta 1; CED; LAP
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human TGF-β1 Transfected Cell Line
Intracellular staining (flow cytometry) (Routinely Tested), Flow cytometry (Tested During Development)
5 µl
AB_11153128
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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562339 Rev. 1
Antibody Details
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TW4-9E7

The TW4-9E7 monoclonal antibody specifically binds to Human Transforming Growth Factor beta-1 (TGF-β1). TGF-β1 is a potent multifunctional cytokine that positively or negatively regulates numerous processes including development, hematopoiesis, tissue remodeling, wound repair, innate and adaptive immunity as well as cancer and autoimmune diseases. TGF-β1 is formed by the enzymatic cleavage of the TGF-β1 propeptide that is encoded by the TGFB1 gene and comprised of the Latency Associated Peptide (LAP) and TGF-β1. Prior to secretion, the dimeric LAP-TGF-β1 propeptide is cleaved resulting in a biologically inactive form of dimeric TGF-β1 that is noncovalently associated with dimeric LAP (latent TGF-β1). This complex may be expressed on the surface of TGF-β1-producing cells or be further processed by proteolytic removal of LAP to release the biologically active mature form of the soluble TGF-β1 homodimer. Many different cell types synthesize TGF-β1 and express specific receptors for it. The TW4-9E7 antibody recognizes both the intracellular latent bound form of TGF-β1 along with the membrane bound form of TGF-β1.

562339 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
496 nm, 566 nm
576 nm
562339 Rev.1
Citations & References
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View product citations for antibody "562339" on CiteAb

Development References (5)

  1. Derynck R, Akhurst RJ. Differentiation plasticity regulated by TGF-beta family proteins in development and disease. Nat Cell Biol. 2007; 9(9):1000-1004. (Biology). View Reference
  2. Derynck R, Jarrett JA, Chen EY, et al. Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells. Nature. 1985; 316(6030):701-705. (Biology). View Reference
  3. Dünker N, Krieglstein K. Targeted mutations of transforming growth factor-beta genes reveal important roles in mouse development and adult homeostasis. Eur J Biochem. 2000; 267(24):6982-6988. (Biology). View Reference
  4. Miyazono K, Hellman U, Wernstedt C, Heldin CH. Latent high molecular weight complex of transforming growth factor beta 1. Purification from human platelets and structural characterization. J Biol Chem. 1988; 263(13):6407-6415. (Biology). View Reference
  5. Oida T, Weiner HL. Overexpression of TGF-β1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-β. J Immunol. 2010; 185(6):3529-3535. (Clone-specific: Flow cytometry). View Reference
View All (5) View Less
562339 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.