PE Mouse Anti-Human CD365 (TIM-1)

This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [753599] or for additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ PE Mouse Anti-Human CD365 (TIM-1)

Name: PE Mouse Anti-Human CD365 (TIM-1)
Brand: BD Pharmingen™
SKU: 564820

Clone 1D12

(RUO)

Flow cytometric analysis of CD365 (TIM-1) expression on human 769-P cells. Cells from the human Jurkat (Acute T cell leukemia, ATCC TIB-152; Left Panel) and 769-P (Renal cell adenocarcinoma, ATCC CRL-1933; Right Panel) cell lines were stained with either PE Mouse IgG1 κ Isotype Control (Cat. No. 554680; dashed line histograms) or PE Mouse Anti-Human CD365 (TIM-1) antibody (Cat. No. 564820; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: CD365; TIM1; TIM; TIMD1; TIMD-1; HAVCR1; HAVCR-1; HAVCR; KIM1; KIM-1

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human TIM-1 Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Workshop Number: X 10-67

RRID: AB_2869615

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

564820 Rev. 2

Antibody Details

1D12

The 1D12 monoclonal antibody specifically binds to CD365, the T-cell immunoglobulin mucin receptor 1 (TIM-1). TIM-1 is expressed on kidney epithelial cells, T cells, and some hematopoietic and non-hematopoietic cells. CD365 (TIM-1) is a type 1 transmembrane glycoprotein that serves as a receptor for hepatitis A virus and is encoded by the HAVCR1 (Hepatitis A virus cellular receptor 1) gene. TIM-1 also serves as a receptor for phosphatidylserine which is exposed on the surface of apoptotic cells. TIM-1 can reportedly mediate the uptake of apoptotic cells through the recognition of phosphatidylserine and thus help maintain tissue homeostasis and self-tolerance. TIM-1 is likewise known as Kidney injury molecule 1 (KIM-1). It is highly expressed by cancerous kidneys, and upregulated in the proximal tubular epithelium and shed into the urine during acute and chronic kidney injury. CD365 (TIM-1) also functions as a costimulatory molecule for immune cells. It is expressed by activated CD4+ T cells and regulates the effector functions (eg, enhanced cytokine production) and survival of differentiated T cells, including those mediating Th2-like immune responses. Other ligands have been described for TIM-1 including TIM-4 and LMIR5 (also known as CD300b) which are expressed by myeloid cells. With respect to disease associations, the HAVCR1 gene has been linked to asthma, allergy, and some autoimmune diseases.

564820 Rev. 2

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

564820 Rev.2

Citations & References

View product citations for antibody "564820" on CiteAb

Development References (7)

Binne LL, Scott ML, Rennert PD. Human TIM-1 associates with the TCR complex and up-regulates T cell activation signals. J Immunol. 2007; 178(7):4342-4350. (Biology). View Reference
Freeman GJ, Casasnovas JM, Umetsu DT, DeKruyff RH. TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.. Immunol Rev. 2010; 235(1):172-89. (Biology). View Reference
Kim HY, Eyheramonho MB, Pichavant M, et al. A polymorphism in TIM1 is associated with susceptibility to severe hepatitis A virus infection in humans. J Clin Invest. 2011; 121(3):1111-1118. (Clone-specific: Blocking, Functional assay). View Reference
Kobayashi N, Karisola P, Pena-Cruz V, et al. TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells. Immunity. 2007; 27(6):927-940. (Immunogen: Blocking, Flow cytometry, Functional assay). View Reference
Manangeeswaran M, Jacques J, Tami C, et al. Binding of hepatitis A virus to its cellular receptor 1 inhibits T-regulatory cell functions in humans. Gastroenterology. 2012; 142(7):1516-1525. (Clone-specific: Blocking, Functional assay). View Reference
Rennert PD. Novel roles for TIM-1 in immunity and infection. Immunol Lett. 2011; 141(1):28-35. (Clone-specific: Blocking). View Reference
Rodriguez-Manzanet R, DeKruyff R, Kuchroo VK, Umetsu DT. The costimulatory role of TIM molecules. Immunol Rev. 2009; 229(1):259-270. (Biology). View Reference

View All (7)

564820 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.