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BD Pharmingen™ FITC Mouse Anti-Human CD80
Clone BB1 (RUO)
Profile of RAJI cells analyzed by flow cytometry.
Profile of RAJI cells analyzed by flow cytometry.
Product Details
BD Pharmingen™
B7-1
Human (QC Testing)
Mouse IgM, κ
Flow cytometry (Routinely Tested)
20 µl
A028, B7.3, BP021
AB_396035
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
555683 Rev. 1
Antibody Details
BB1
Reacts with a 60 kD transmembrane glycoprotein which was clustered as CD80 in the Fifth International Workshop on Human Differentiation Antigens. There have been several recent studies reporting discrepancies in the expression and function of CD80 when the BB1 clone is used compared to other anti-CD80 monoclonals (e.g., L307.4). Caution should therefore be used in the interpretation of data generated using BB1, as reports indicate that BB1 may recognize, in addition to CD80, CD74 and even possibly another as yet undefined molecule. CD80, a member of the Ig supergene family, is expressed on activated B cells, macrophages, and dendritic cells. It is the ligand for two molecules expressed on T cells: CD28 and CD152 (CTLA-4). CD80 is also expressed in activated CD4+ and CD8+ T cells, appearing late after activation, suggesting that activated T cells may be capable of autocrine costimulation via the CD28 activation pathway. The binding of CD28 molecule by CD28 mAb or by CD80 antigen results in T-cell activation and a signal for IL-2 production.
555683 Rev. 1
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
494 nm
518 nm
555683 Rev.1
Citations & References
View product citations for antibody "555683" on CiteAb
Development References (6)
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Azuma M, Yssel H, Phillips JH, Spits H, Lanier LL. Functional expression of B7/BB1 on activated T lymphocytes. J Exp Med. 1993; 177(3):845-850. (Clone-specific). View Reference
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Behrens L, Kerschensteiner M, Misgeld T, Goebels N, Wekerle H, Hohlfeld R. Human muscle cells express a functional costimulatory molecule distinct from B7.1 (CD80) and B7.2 (CD86) in vitro and in inflammatory lesions. J Immunol. 1998; 161(11):5943-5951. (Clone-specific). View Reference
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Freeman GJ, Cardoso AA, Boussiotis VA, et al. The BB1 monoclonal antibody recognizes both cell surface CD74 (MHC class II-associated invariant chain) as well as B7-1 (CD80), resolving the question regarding a third CD28/CTLA-4 counterreceptor. J Immunol. 1998; 161(6):2708-2715. (Clone-specific). View Reference
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Koulova L, Clark EA, Shu G, Dupont B. The CD28 ligand B7/BB1 provides costimulatory signal for alloactivation of CD4+ T cells. J Exp Med. 1991; 173(3):759-762. (Clone-specific). View Reference
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Schwartz RH. Costimulation of T lymphocytes: the role of CD28, CTLA-4, and B7/BB1 in interleukin-2 production and immunotherapy. Cell. 1992; 71(7):1065-1068. (Clone-specific: Activation). View Reference
555683 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.