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BD Pharmingen™ FITC Mouse Anti-Human CD77
Clone 5B5 (RUO)
Profile of Ramos cell line analyzed by flow cytometry
Profile of Ramos cell line analyzed by flow cytometry
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse BALB/c IgM, κ
Initially Adherent Human Peripheral Blood Cells
Flow cytometry (Routinely Tested)
20 µl
VI CD77.5
AB_394165
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
551353 Rev. 4
Antibody Details
5B5
The 5B5 monoclonal antibody specifically binds to human CD77, a small neutral glycosphingolipid also referred to as globotriaosylceramide (Gb3), Burkitt 's lymphoma associated antigen (BLA), ceramide trihexoside (CTH), and Pk blood group antigen. CD77 is expressed on a subset of germinal center B-lymphocytes including centroblasts that are found with greater frequency in tonsil and mucosal lymphoid tissues and less frequently in peripheral blood, bone marrow and spleen. It has been described as a receptor for Shiga Toxin, produced by Shigella dysenteriae, and Verotoxin 1 (VT1), produced by some strains of E. coli. CD19 has been reported as a ligand for CD77. This interaction may function in adhesion and signal transduction at a specific stage of B cell differentiation. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
551353 Rev. 4
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
494 nm
518 nm
551353 Rev.4
Citations & References
View product citations for antibody "551353" on CiteAb
Development References (4)
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Fyfe G, Cebra-Thomas JA, Mustain E, Davie JM, Alley CD, Nahm MH. Subpopulations of B lymphocytes in germinal centers. J Immunol. 1987; 139(7):2187-2194. (Biology). View Reference
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Lagresle C, Bella C, Daniel PT, Krammer PH, Defrance T. Regulation of germinal center B cell differentiation. Role of the human APO-1/Fas (CD95) molecule. J Immunol. 1995; 154(11):5746-5756. (Biology). View Reference
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Maloney MD, Lingwood CA. CD19 has a potential CD77 (globotriaosyl ceramide)-binding site with sequence similarity to verotoxin B-subunits: implications of molecular mimicry for B cell adhesion and enterohemorrhagic Escherichia coli pathogenesis. J Exp Med. 1994; 180(1):191-201. (Biology). View Reference
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Mangeney M, Rousselet G, Taga S, Tursz T, Wiels J. The fate of human CD77+ germinal center B lymphocytes after rescue from apoptosis. Mol Immunol. 1995; 32(5):333-339. (Biology). View Reference
551353 Rev. 4
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.