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Profile of peripheral blood monocytes analyzed by flow cytometry
BD Pharmingen™ FITC Mouse Anti-Human CD329 (Siglec-9)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The E10-286 monoclonal antibody specifically binds to CD329. CD329 is also known as Sialic acid-binding Ig-like lectin 9 (Siglec-9), formerly known as Siglec-Y, which is expressed on peripheral blood monocytes, granulocytes and NK cells. Siglecs (sialic acid/immunolgobulin/lectin) are a family of I-type lectins binding to sialic acids on the cell surface. They are a family of carbohydrate-binding proteins within the immunoglobulin superfamily. Siglecs are all integral membrane proteins with extracellular domains consisting of unusual N-termial V-set Ig domains, followed by variable numbers of C2-set Ig domains.
Development References (2)
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Powell LD, Varki A. I-type lectins. J Biol Chem. 1995; 270(24):14243-14246. (Biology). View Reference
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Varki A, Chrispeels MJ. Varki, A., Esko, J. Cummings, R. Ajit Varki .. et al. ; with contributions from Maarten Chrispeels .. et al., ed. Essentials of glycobiology. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press; 1999:101.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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