Dear Customer, If you experience any text readability or display issues on our website, please update your web browser to the latest version
See More
BD OptiBuild™ BV421 Mouse Anti-Rat IgG1/IgG2a
Clone G28-5 (RUO)
Flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Rat IgG1/IgG2a antibody (Cat. No. 743849; solid line histogram) for secondary staining of BALB/c splenocytes first stained with either Purified Isotype Control (dotted line histograms) or with Purified Rat IgG2a Anti-Mouse CD8a (solid line; left panel) or with Purified Rat IgG1 Anti-Mouse CD8b.2 antibody (solid line; right panel). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Rat IgG1/IgG2a antibody (Cat. No. 743849; solid line histogram) for secondary staining of BALB/c splenocytes first stained with either Purified Isotype Control (dotted line histograms) or with Purified Rat IgG2a Anti-Mouse CD8a (solid line; left panel) or with Purified Rat IgG1 Anti-Mouse CD8b.2 antibody (solid line; right panel). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Rat IgG1/IgG2a antibody (Cat. No. 743849; solid line histogram) for secondary staining of BALB/c splenocytes first stained with either Purified Isotype Control (dotted line histograms) or with Purified Rat IgG2a Anti-Mouse CD8a (solid line; left panel) or with Purified Rat IgG1 Anti-Mouse CD8b.2 antibody (solid line; right panel). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Rat IgG1/IgG2a antibody (Cat. No. 743849; solid line histogram) for secondary staining of BALB/c splenocytes first stained with either Purified Isotype Control (dotted line histograms) or with Purified Rat IgG2a Anti-Mouse CD8a (solid line; left panel) or with Purified Rat IgG1 Anti-Mouse CD8b.2 antibody (solid line; right panel). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Product Details
BD OptiBuild™
IgG1/IgG2a; Ig gamma-1/2a; Immunoglobulin gamma 1/2a
Rat (Tested in Development)
Mouse IgG2a
Pooled Rat IgG
Flow cytometry (Qualified)
0.2 mg/ml
AB_2741802
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
Companion Products
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.1 mg
Cat No.
553141
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Brilliant Stain Buffer RUO
Size
100 Tests
Cat No.
563794
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
BV421 Mouse IgG2a, k Isotype Control RUO
Size
50 µg
Cat No.
562439
743849 Rev. 2
Antibody Details
G28-5
The G28-5 monoclonal antibody reacts specifically with rat IgG1 and IgG2a. It does not react with other Ig isotypes. A suspension of pooled rat IgG1 was used as the source of immunogen.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
743849 Rev. 2
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
743849 Rev.2
Citations & References
View product citations for antibody "743849" on CiteAb
No Citations Are Available for this Product
743849 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.