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Biotin Mouse Anti-Human LT-α

BD Pharmingen™ Biotin Mouse Anti-Human LT-α

Name: Biotin Mouse Anti-Human LT-α
Brand: Biotin Mouse Anti-Human LT-α
SKU: 554555

Clone 359-81-11 (RUO)

Product Details

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1

Immunogen: Recombinant Human LT-α (TNF-β)

Application: ELISA Detection (Routinely Tested), Flow cytometry, Neutralization (Tested During Development), Western blot (Not Recommended)

Concentration: 0.5 mg/ml

RRID: AB_395477

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

1. ELISA Detection: The biotinylated 359-81-11 antibody (Cat. No. 554555) is useful as a detection antibody for a sandwich ELISA for measuring human LT-α protein levels. Biotinylated 359-81-11 antibody can be paired with the purified 359-238-8 antibody (Cat. No. 551222) as the capture antibody, with recombinant LT-α (Cat. No. 554619) as the standard. Biotinylated 359-81-11 antibody should be titrated (0.5 - 2.0 µg/ml) to determine optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of human LT-α ranging from ~2,000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring LT-α in serum or plasma our LT-α BD OptEIA™ set (Cat. No. 550995) is specially formulated and recommended.

2. Neutralization: The NA/LE™ 359-81-11 antibody (Cat. No. 554553) has been reported to be useful for neutralization of human LT-α bioactivity. A suitable NA/LE mouse IgG1 isotype control to match the 359-81-11 antibody is the 107.3 antibody, (Cat. No. 554721).

3. IF/Flow: The 359-81-11 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate LT-α producing cells within mixed cell populations. The PE-conjugated 359-81-11 antibody (Cat. No. 554556) is especially suitable for these studies.

4. Western Blot: The 359-81-11 antibody is not recommended for Western blotting.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

554555 Rev. 1

Antibody Details

359-81-11

The 359-81-11 antibody reacts with human Lymphotoxin-α (LT-α, also known as tumor necrosis factor-β or TNF-β). The immunogen used to generate the 359-81-11 hybridoma was recombinant human LT-α. This is a neutralizing antibody.

554555 Rev. 1

Format Details

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

Format: Biotin

554555 Rev.1

Citations & References

View product citations for antibody "554555" on CiteAb

Development References (4)

Callard R, Gearing A. Callard R, Gearing A. The Cytokine Facts Book. San Diego: Academic Press; 1994.
Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Methodology). View Reference
Meager A, Parti S, Leung H, Peil E, Mahon B. Preparation and characterization of monoclonal antibodies directed against antigenic determinants of recombinant human tumour necrosis factor (rTNF). Hybridoma. 1987; 6(3):305-312. (Clone-specific: ELISA, Neutralization). View Reference
Meager A, Parti S, Leung H, et al. A two-site sandwich immunoradiometric assay of human lymphotoxin with monoclonal antibodies and its applications. J Immunol Methods. 1987; 104(2):31-42. (Clone-specific: ELISA, Neutralization). View Reference

View All (4)

554555 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.