Dear Customer, If you experience any text readability or display issues on our website, please update your web browser to the latest version
See More
Product Details
BD Pharmingen™
Rat (QC Testing)
Human Bcl-X aa. 46-66 synthetic peptide
ELISA (Routinely Tested), Flow cytometry, Immunocytochemistry, Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Immunohistochemistry-zinc-fixed (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The polyclonal antibody was purified from antiserum by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
Biotin Goat Anti-Rat Ig RUO
Size
0.5 mg
Cat No.
559286
Biotin Mouse Anti-Rat IgG2b RUO
Size
1 ML
Cat No.
550327
Biotin Mouse Anti-Rat IgM RUO
Size
0.5 mg
Cat No.
553886
559286 Rev. 21
Antibody Details
Poly1271
This polyclonal antibody reacts with whole molecule rat IgG (heavy and light chains) and may also react with the light chains of other rat immunoglobulins. The antibody has been reported to be reactive in ELISA with rat IgG, IgM, and IgA. In addition, some cross-reactivity with mouse IgG, some hamster Ig and minimal cross-reactivity with human Ig has been reported. Investigators experiencing cross-reactivity with rat IgM may wish to consider using the following alternatives: Biotin Mouse Anti-Rat IgM (Cat. No. 553886) or Biotin Mouse Anti-Rat Ig, κ light chain (Cat. No. 553871). Minimal cross-reaction to mouse, human, bovine, horse and rabbit non-immunoglobulin serum proteins are expected.
When using rat IgG2b primary antibodies, Biotin Mouse Anti-Rat IgG2b (Cat. No. 550327) is also available as an alternate. When using rat IgM primary antibodies, Mouse Anti-Rat/Hamster Ig κ light chain (Cat. No. 550336) is also available as an alternative.
559286 Rev. 21
Format Details
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
559286 Rev.21
Citations & References
View product citations for antibody "559286" on CiteAb
No Citations Are Available for this Product
559286 Rev. 21
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.