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      APC Rat anti-Mouse CD184
      APC Rat anti-Mouse CD184
      Expression of CD184 on mouse splenocytes. Mouse splenocytes were simultaneously stained with APC Rat anti-Mouse CD184 (Cat. No. 558644; Right Panel) or APC Rat IgG2b, κ Isotype Control(Cat. No. 556924; Left Panel) and FITC Rat Anti-Mouse CD4 (Cat. No. 553047; Both Panels). Flow cytometry was performed on a BD FACSCalibur™ Flow Cytometry System.
      APC Rat anti-Mouse CD184
      Expression of CD184 on mouse splenocytes. Mouse splenocytes were simultaneously stained with APC Rat anti-Mouse CD184 (Cat. No. 558644; Right Panel) or APC Rat IgG2b, κ Isotype Control(Cat. No. 556924; Left Panel) and FITC Rat Anti-Mouse CD4 (Cat. No. 553047; Both Panels). Flow cytometry was performed on a BD FACSCalibur™ Flow Cytometry System.
      Product Details
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      BD Pharmingen™
      CXCR4, C-X-C chemokine receptor type 4; Fusin; LESTR; PB-CKR; Sdf1r
      Mouse (QC Testing)
      Rat IgG2b, κ
      GST-NCXCR4 fusion protein
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      12767
      AB_1645219
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
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      Recommended Assay Procedures

      Clone 2B11/CXCR4 has been reported to perform optimally when allowed to stain for 45 minutes.  Please refer to http://www.bdbiosciences.com/support/resources/ for additional resources and protocols.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. An isotype control should be used at the same concentration as the antibody of interest.
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      558644 Rev. 2
      Antibody Details
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      2B11/CXCR4

      The 2B11/CXCR4 monoclonal antibody specifically reacts with mouse CD184, which is also known as CXC chemokine receptor, CXCR4.  CXCR4 (previously known as Fusin and LESTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12.  Mouse CXCR4 shows 91% homology at amino acid level with human CXCR4. CXCR4 is widely expressed by hematopoietic and non-hematopoietic cell types including neutrophils, monocytes, T cells, B cells, CD34-positive progenitor cells, endothelial cells, neurons and astrocytes.  In the thymus CXCR4 is restricted to CD4+CD8+ cells, while in the spleen, predominant expression is found on B lymphocytes.  Human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry.  The mouse CXCR4 gene has been mapped to chromosome 1.

      558644 Rev. 2
      Format Details
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      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      558644 Rev.2
      Citations & References
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      View product citations for antibody "558644" on CiteAb

      Development References (10)

      1. Bleul CC, Farzan M, Choe H, et al. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature. 1996; 382(6594):829-833. (Biology). View Reference
      2. Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR. The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.. Proc Natl Acad Sci U S A. 1997; 94(5):1925-1930. (Biology). View Reference
      3. Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996; 272(5263):872-877. (Biology). View Reference
      4. Forster R, Kremmer E, Schubel A, et al. Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J Immunol. 1998; 160(3):1522-1531. (Immunogen). View Reference
      5. Gupta SK, Lysko PG, Pillarisetti K, Ohlstein E, Stadel JM. Chemokine receptors in human endothelial cells. Functional expression of CXCR4 and its transcriptional regulation by inflammatory cytokines. J Biol Chem. 1998; 273(7):4282-4287. (Biology). View Reference
      6. Heesen M, Berman MA, Benson JD, Gerard C, Dorf ME. Cloning of the mouse fusin gene, homologue to a human HIV-1 co-factor. J Immunol. 1996; 157(12):5455-5460. (Biology). View Reference
      7. Hesselgesser J, Halks-Miller M, DelVecchio V, et al. CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons. Curr Biol. 1997; 7(2):112-121. (Biology). View Reference
      8. Loetscher M, Geiser T, O'Reilly T, Zwahlen R, Baggiolini M, Moser B. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. J Biol Chem. 1994; 269(1):232-237. (Biology). View Reference
      9. Oberlin E, Amara A, Bachelerie F, et al. The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. Nature. 1996; 382(6594):833-835. (Biology). View Reference
      10. Schabath R, Muller G, Schubel A, Kremmer E, Lipp M, Forster R. The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation. J Leukoc Biol. 1999; 66(6):996-1004. (Biology). View Reference
      View All (10) View Less
      558644 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.