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      Alexa Fluor® 647 Mouse Anti-Human CRTAM (CD355)
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      Alexa Fluor® 647 Mouse Anti-Human CRTAM (CD355)
      Two-color flow cytometric analysis of CRTAM (CD355) expression on activated human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 4 hours with Phorbol 12-Myristate 13-Acetate (PMA; 10 ng/ml) and Ionomycin (1 µg/ml). The cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD8 antibody (Cat.No. 562428) and either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CRTAM (CD355) antibody (Cat. No. 566645; Right Plot) at 1 µg/test. Two-color flow-cytometric pseudocolor plots showing the correlated expression of CD8 versus CRTAM (CD355) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
      Alexa Fluor® 647 Mouse Anti-Human CRTAM (CD355)
      Two-color flow cytometric analysis of CRTAM (CD355) expression on activated human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 4 hours with Phorbol 12-Myristate 13-Acetate (PMA; 10 ng/ml) and Ionomycin (1 µg/ml). The cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD8 antibody (Cat.No. 562428) and either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CRTAM (CD355) antibody (Cat. No. 566645; Right Plot) at 1 µg/test. Two-color flow-cytometric pseudocolor plots showing the correlated expression of CD8 versus CRTAM (CD355) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      CRTAM; class-I MHC-restricted T-cell-associated molecule; cytotoxic and regulatory T-cell molecule
      Human (QC Testing)
      Mouse BALB/c IgG2a, κ
      Human CRTAM Transfected Cell Line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2869804
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      566645 Rev. 1
      Antibody Details
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      Cr24.1

      The CR24.1 monoclonal antibody specifically recognizes Class I MHC-restricted T cell-associated molecule (CRTAM) which is also known as CD355, Class I-restricted T cell-associated molecule, or Cytotoxic and regulatory T-cell molecule. CRTAM (CD355) is a type I transmembrane glycoprotein that belongs to the Nectin family of cell adhesion molecules within the Ig superfamily. It is expressed on activated NK cells, NKT cells, γδ T cells, CD8+ T cells, and by Purkinje neurons of the cerebellum. CRTAM can exist as a homodimer or as a heterodimer when bound to its ligand, Nectin-like molecule 2 (Necl-2), which is encoded by TSLC1 (tumor suppressor gene tumor suppressor in lung cancer-1). Necl-2-CRTAM interactions play important roles in cellular adhesion and migration and can augment IFN-γ production by CD8+ T cells and cytotoxicity by NK cells.

              

      566645 Rev. 1
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      566645 Rev.1
      Citations & References
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      View product citations for antibody "566645" on CiteAb

      Development References (5)

      1. Boles KS, Barchet W, Diacovo T, Cella M, Colonna M. The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM.. Blood. 2005; 106(3):779-86. (Immunogen: Blocking, Flow cytometry). View Reference
      2. Dessarthe B, Thedrez A, Latouche JB, et al. CRTAM receptor engagement by Necl-2 on tumor cells triggers cell death of activated Vγ9Vδ2 T cells.. J Immunol. 2013; 190(9):4868-76. (Biology). View Reference
      3. Galibert L, Diemer GS, Liu Z, et al. Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a ligand for class-I-restricted T-cell-associated molecule.. J Biol Chem. 2005; 280(23):21955-64. (Biology). View Reference
      4. Kennedy J, Vicari AP, Saylor V, et al. A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM).. J Leukoc Biol. 2000; 67(5):725-34. (Biology). View Reference
      5. Patiño-Lopez G, Hevezi P, Lee J, et al. Human class-I restricted T cell associated molecule is highly expressed in the cerebellum and is a marker for activated NKT and CD8+ T lymphocytes.. J Neuroimmunol. 2006; 171(1-2):145-55. (Biology). View Reference
      View All (5) View Less
      566645 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.