BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human CD49b
Clone 12F1 (RUO)
Flow cytometric analysis of CD49b expression on human peripheral blood platelets. Platelets were stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CD49b antibody (Cat. No. 564118; solid line histogram). Fluoresence histograms depicting CD49b (or Ig Isotype control) expression were derived from gated events with the side and forward light-scatter characteristics of viable platelets. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of CD49b expression on human peripheral blood platelets. Platelets were stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CD49b antibody (Cat. No. 564118; solid line histogram). Fluoresence histograms depicting CD49b (or Ig Isotype control) expression were derived from gated events with the side and forward light-scatter characteristics of viable platelets. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
Integrin α2 chain; ITGA2; VLA-2 alpha; Platelet GPIa; Collagen receptor; BR
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human PBMC and Platelets
Flow cytometry (Routinely Tested)
5 µl
V S220
3673
AB_2738604
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO
Size
100 Tests
Cat No.
557715
564118 Rev. 2
Antibody Details
12F1
The 12F1 monoclonal antibody specifically binds to CD49b. CD49b is also known as the integrin α2 chain (VLA-α2), a member of the integrin family of extracellular matrix and cell-cell adhesion receptors. VLA-α2 is non-covalently associated with VLA-β1 chain (CD29) in the VLA-2 complex. α2 is expressed on numerous cell types including activated T cells, platelets, and long-term cultivated T cells. α2β1 is a well-established receptor for collagen. This antibody is useful for studies of integrin expression and function.
564118 Rev. 2
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564118 Rev.2
Citations & References
View product citations for antibody "564118" on CiteAb
Development References (6)
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Hemler ME, Kawaguchi S, Bodorova J. CD49b cluster repoty. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1615-1616.
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Hemler ME. VLA proteins in the integrin family: structures, functions, and their role on leukocytes. Annu Rev Immunol. 1990; 8:365-400. (Biology). View Reference
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Pischel KD, Bluestein HG, Woods VL, Jr. Platelet glycoproteins Ia, Ic, and IIa are physicochemically indistinguishable from the very late activation antigens adhesion-related proteins of lymphocytes and other cell type. J Clin Invest. 1988; 81(2):505-513. (Clone-specific: Immunoprecipitation, Radioimmunoassay). View Reference
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Pischel KD, Hemler ME, Huang C, Bluestein HG, Woods VL, Jr. Use of the monoclonal antibody 12F1 to characterize the differentiation antigen VLA-2. J Immunol. 1987; 138(1):226-233. (Clone-specific: Flow cytometry, Immunoprecipitation). View Reference
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
564118 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.