BD Pharmingen™ Alexa Fluor® 488 Mouse anti-Sox2
Clone O30-678 (RUO)
Analysis of Sox2 on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD™ Phosflow Perm buffer III (Cat. No. 558050) and stained with matching concentrations of either Alexa Fluor® 488 Mouse IgG1, κ isotype control (dashed line, Cat. No. 557721) or Alexa Fluor® 488 Mouse anti-Sox2 monoclonal antibody (solid line). Histograms were derived from gated events based on light scattering characteristics of the H9 cell line. Flow cytometry was performed on a BD LSR™ II flow cytometry system.
Immunoflourescent staining of human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI), passage 33 grown on irradiated mouse embryonic fibroblasts, were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD™ Phosflow Perm Buffer III (Cat No. 558050), and stained with Alexa Fluor® 488 Mouse anti-Sox2 monoclonal antibody (pseudo colored green) at 1.2 µg/mL. Counter-staining of cell nuclei was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software.
Analysis of Sox2 on human ES cell-derived neural stem cells (NSC). NSC derived from H9 human ES cells (WiCell, Madison, WI) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD™ Phosflow Perm buffer III (Cat. No. 558050), and stained with matching concentrations of either Alexa Fluor® 488 Mouse IgG1, κ isotype control (dashed line, Cat. No. 557721) or Alexa Fluor® 488 Mouse Anti-Sox2 monoclonal antibody (solid line). Histograms were derived from gated events based on light scattering characteristics of the H9-derived NSC. Flow cytometry was performed on a BD LSR™ II flow cytometry system.
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Analysis of Sox2 on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD™ Phosflow Perm buffer III (Cat. No. 558050) and stained with matching concentrations of either Alexa Fluor® 488 Mouse IgG1, κ isotype control (dashed line, Cat. No. 557721) or Alexa Fluor® 488 Mouse anti-Sox2 monoclonal antibody (solid line). Histograms were derived from gated events based on light scattering characteristics of the H9 cell line. Flow cytometry was performed on a BD LSR™ II flow cytometry system.
Immunoflourescent staining of human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI), passage 33 grown on irradiated mouse embryonic fibroblasts, were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD™ Phosflow Perm Buffer III (Cat No. 558050), and stained with Alexa Fluor® 488 Mouse anti-Sox2 monoclonal antibody (pseudo colored green) at 1.2 µg/mL. Counter-staining of cell nuclei was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software.
Analysis of Sox2 on human ES cell-derived neural stem cells (NSC). NSC derived from H9 human ES cells (WiCell, Madison, WI) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD™ Phosflow Perm buffer III (Cat. No. 558050), and stained with matching concentrations of either Alexa Fluor® 488 Mouse IgG1, κ isotype control (dashed line, Cat. No. 557721) or Alexa Fluor® 488 Mouse Anti-Sox2 monoclonal antibody (solid line). Histograms were derived from gated events based on light scattering characteristics of the H9-derived NSC. Flow cytometry was performed on a BD LSR™ II flow cytometry system.
Product Details
BD Pharmingen™
Human (QC Testing), Mouse (Reactivity Confirmed in Development)
Mouse CD, also known as Charles River SD (outbred) IgG1, κ
Human Sox2 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging, Immunofluorescence (Tested During Development)
5 µl
AB_10894382
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
Alexa Fluor® 488 Mouse IgG1 κ Isotype Control RUO
Size
100 Tests
Cat No.
557721
561593 Rev. 1
Antibody Details
O30-678
The monoclonal antibody O30-678 recognizes the Sox2 transcription factor. Sox2 [SRY (sex determining region Y)-box 2] is a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox2 is required for the maintenance of the undifferentiated state of pluripotent stem cells. Complexes of Sox2 with the homeobox transcription factors Oct3/4 and/or Nanog bind to the promoters of a network of genes that are involved in the maintenance of pluripotency and self renewal in stem cells. Sox2 is also a marker of neural stem cells during embryonic development and in the adult brain. The O30-678 antibody recognizes both human and mouse Sox2 proteins.
561593 Rev. 1
Format Details
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
561593 Rev.1
Citations & References
View product citations for antibody "561593" on CiteAb
Development References (3)
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Boyer LA, Lee TI, Cole MF, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 2005; 122:947-956. (Biology). View Reference
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Pan G, Thomson JA. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 2007; 17:42-49. (Biology). View Reference
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Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-676. (Biology). View Reference
561593 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.