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This is a suggested alternate SKU for 564281.
Product Details
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BD Pharmingen™
Anti-Keyhole Limpet Hemocyanin (KLH)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
Trinitrophenyl–Keyhole Limpet Hemocyanin
Flow cytometry, Isotype control (Routinely Tested)
0.2 mg/ml
AB_1727590
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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560720 Rev. 1
Antibody Details
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B39-4

The B39-4 hybridoma was generated using cells from a rat that was immunized with the hapten-carrier conjugate, trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH). The B39-4 antibody is specific for KLH. It has an IgG2a, λ isotype and can serve as a immunoglobulin isotype control.  

560720 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
651 nm
660 nm
560720 Rev.1
Citations & References
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View product citations for antibody "560720" on CiteAb
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560720 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.