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PE Mouse Anti-Human IL-6
Product Details
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BD FastImmune™
Human
Mouse IgG1
Recombinant human IL-6 receptor antagonist
Intracellular staining (flow cytometry)
1.5 μg/mL
20 μL
3569
AB_400442
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340527 Rev. 1
Antibody Details
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AS12

The Anti-Hu–IL-6 antibody, clone AS12, is derived from the fusion of P3X63.Ag8.653 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-6.

The Anti-Human Interleukin-6 (Anti-Hu–IL-6) antibody recognizes a 21- to 29-kilodalton (kDa) polypeptide.

340527 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340527 Rev.1
Citations & References
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Development References (24)

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  2. Barlogie B, Epstein J, Selvanayagam P, Alexanian R. Plasma cell myeloma: New biological insights and advances in therapy. Blood. 1989; 73:865-879. (Biology).
  3. Birx DL, Redfield RR, Tencer K, et al. Induction of interleukin-6 during human immunodeficiency virus infection. Blood. 1990; 76:2303-2310. (Biology).
  4. Breen EC, Rezai AR, Nakajuma K, et al. Infection with HIV is associated with elevated IL-6 levels and production. J Immunol. 1990; 144:480-484. (Biology).
  5. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  6. Clinical and Laboratory Standards Institute. 2005. (Biology).
  7. Dokter WHA, Koopmans SB, Vellenga E. Effects of IL-10 and IL-4 on LPS-induced transcription factors (AP-1, NF-IL6 and NF-κB) which are involved in IL-6 regulation. Leukemia. 1996; 10:1308-1316. (Biology).
  8. Feldmann M, Brennan FM, Maini RN. Rheumatoid arthritis. Cell. 1996; 85:307-310. (Biology).
  9. Feldmann M, Brennan FM, Maini RN. Role of cytokines in rheumatoid arthritis. Ann Rev Immunol. 1996; 14:397-440. (Biology).
  10. Gauldie J, Richards C, Harnish D, Lansdorp P, Baumann H. Interferon β 2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells. Proc Natl Acad Sci USA. 1987; 84:7251-7256. (Biology).
  11. Hibi M, Murakami M, Saito M, Hirano T, Taga T, Kishimoto T. Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell. 1990; 63(6):1149-1157. (Biology). View Reference
  12. Iwakura Y, Tosu M, Yoshida E, et al. Induction of inflammatory arthropathy resembling rheumatoid arthritis in mice transgenic for HTLV-1. Science. 1991; 253:1026-1028. (Biology).
  13. Kishimoto T, Akira S, Taga T. Interleukin-6 and its receptor: a paradigm for cytokines. Science. 1992; 258(5082):593-597. (Biology). View Reference
  14. Kishimoto T. The biology of interleukin-6. Blood. 1989; 74:43840. (Biology).
  15. Kohase M, May LT, Tamm I, Vilcek J, Sehgal PB. A cytokine network in human diploid fibroblasts: Interactions of β -interferons, tumor necrosis factor, platelet-derived growth factor, and interleukin-1. Mol Cell Biol. 1987; 7:273-280. (Biology).
  16. Koike K, Nakahata T, Takagi M, et al. Synergism of BSF-2/interleukin 6 and interleukin 3 on development of multipotential hemopoietic progenitors in serum-free culture. J Exp Med. 1988; 168:879-890. (Biology).
  17. Libermann TA, Baltimore D. Activation of interleukin-6 gene expression through the NF-κB transcription factor. Mol Cell Biol. 1990; 10:2327-2334. (Biology).
  18. Lotz M, Jirik F, Kabouridis P, et al. B cell stimulating factor 2/interleukin 6 is a costimulant for human thymocytes and T lymphocytes. J Exp Med. 1988; 167:1253-1258. (Biology).
  19. May LT, Ghrayeb J, Santhanam U, et al. Synthesis and secretion of multiple forms of β2-interferon/B-cell differentiation factor 2/hepatocyte-stimulating factor by human fibroblasts and monocytes. J Biol Chem. 1988; 263:7760-7765. (Biology).
  20. Muraguchi A, Hirano T, Tang B, et al. The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells. J Exp Med. 1988; 167:332-344. (Biology).
  21. Muraguchi A, Kishimoto T, Miki Y, et al. T-cell replacing factor–(TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF. J Immunol. 1981; 127:412-416. (Biology).
  22. Van Damme J, Opdenakker G, Simpson RJ, et al. Identification of the human 26-kD protein, interferon β 2 (IFN- β2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor. J Exp Med. 1987; 165:914-919. (Biology).
  23. Van Snick, J. Interleukin 6: an overview. Ann Rev Immunol. 1990; 8:253-278. (Biology).
  24. Zhang Y, Broser M, Rom WN. Activation of the interleukin 6 gene by mycobacterium tuberculosis or lipopolysaccharide is mediated by nuclear factors NF-IL6 and NF-κB. Proc Natl Acad Sci USA. 1994; 91:2225-2229. (Biology).
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340527 Rev. 1

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