PE Mouse Anti-Human CD134

BD™ PE Mouse Anti-Human CD134

Name: PE Mouse Anti-Human CD134
Brand: BD™
SKU: 340420

Clone L106

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: OX40; OX 40; OX40L Receptor; TNFRSF4; ACT35; TXGP1L; OX40L Receptor; IMD16

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Phytohemagglutinin-activated T lymphocytes

Application: Flow cytometry

Vol. Per Test: 20 μL

Entrez Gene ID: 7293

RRID: AB_400027

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The PE conjugate is supplied in 1.0 mL of PBS containing gelatin and 0.1% sodium azide. You may wish to titer the reagent for use in your specific application. Refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340420 Rev. 1

Antibody Details

L106

CD134, clone L106, is derived from hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with phytohemagglutinin-activated T lymphocytes.

CD134 (Anti-Human Ox 40) recognizes a 50-kdalton (kDa) antigen that is present on activated T lymphocytes. By sequence analysis, it is related to the rat monoclonal antibody Ox 40, a member of the nerve growth factor superfamily that also includes CD27, CD30, CD40, CD95 (Fas), and the tumor necrosis factors alpha and beta (CD120a and CD120b).

340420 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

340420 Rev.1

Citations & References

View product citations for antibody "340420" on CiteAb

Development References (7)

Barclay AN, Birkeland ML, Brown MH, et al. 1993.
Fanslow WC, Clifford KN, Zappone J, Sorensen R, Gaum P. Involvement of Ox40 interaction in murine cardiac allograft rejection. 1995; 575a. (Biology).
Godfrey W, Buck D, Harara M, Benike C, Engleman E. Studies of the L106 protein and its gene: Evidence that L106 and Ox 40 are homologous. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1157-1160.
Godfrey WR, Fagnoni FF, Harara MA, Buck D, Engleman EG. Identification of a human OX-40 ligand, a costimulator of CD4+ T cells with homology to tumor necrosis factor. J Exp Med. 1994 August; 180(2):757-762. (Biology). View Reference
Mallett S, Fossum S, Barclay AN. Characterization of the MRC OX40 antigen of activated CD4 positive T lymphocytes--a molecule related to nerve growth factor receptor. EMBO J. 1990; 9(4):1063-1068. (Biology). View Reference
Paterson DJ, Jefferies WA, Green JR, et al. Antigens of activated rat lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T cell blasts. Mol Immunol. 1993; 24:1282. (Biology).
Tittle TV, Steinkeler C, Weinberg A, Maziarz R. The expression of the T cell activation antigen, Ox-40, in GVHD. Blood. 1995; 86(10):575a-576a. (Biology).

View All (7)

340420 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.