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CD64 BV605
Product Details
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BD Horizon™
FCGR1; FcRI; Fc-gamma RI; IgG Fc Receptor I; High affinity IgG FcR1
Human
Mouse BALB/c IgG1, κ
Human Rheumatoid synovial fluid cells and fibronectin-purified monocytes
Flow cytometry
200 μg/mL
5 μL
VI MA36
2209
ASR


663991 Rev. 1
Antibody Details
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10.1

The CD64 antibody, clone 10.1, is derived from the hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells isolated from a BALB/c mouse that was immunized first with 2 x 107 rheumatoid synovial fluid cells and on subsequent occasions with 1.5 x 107 fibronectin-purified human monocytes obtained from pools of blood group–matched donors.

CD64 recognizes the 72-kilodalton (kDa) human FcγRI that can bind monomeric IgG.

663991 Rev. 1
Format Details
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BV605
The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm. BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter). The acceptor dye can be excited by the yellow-green (561-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV605
Violet 405 nm
407 nm
605 nm
663991 Rev.1
Citations & References
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663991 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.