CD62P PE

BD™ CD62P PE

Name: CD62P PE
Brand: BD™
SKU: 654976

Clone AC1.2

(ASR)

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Product Details

Brand: BD™

Alternative Name: SELP; PSEL; P-selectin; PADGEM; LECAM3; GMP-140; GRMP; GMP140

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Activated Human Platelets

Application: Flow cytometry

Concentration: 1.5 μg/mL

Vol. Per Test: 20 μL

Workshop Number: V S058

Entrez Gene ID: 6403

Regulatory Status: ASR

654976 Rev. 1

Antibody Details

AC1.2

The CD62P antibody, clone AC1.2, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with activated platelets.

The CD62P antibody recognizes a molecule known as platelet activation-dependent granule-external membrane (PADGEM) protein or granule membrane protein (GMP-140), which is a 140-kilodalton (kDa) single-chain polypeptide. The CD62P antigen is a member of the selectin family of adhesion molecules and mediates the adhesion of activated platelets to neutrophils and monocytes in hemostasis.

654976 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

654976 Rev.1

Citations & References

View product citations for antibody "654976" on CiteAb

Development References (12)

Ault KA, Rinder HM, Mitchell JG, Rinder CS, Lambrew CT, Hillman RS. Correlated measurement of platelet release and aggregation in whole blood. Cytometry. 1989; 10:448-455. (Biology).
Berman CL, Yeo EL, Wencel-Drake JD, Furie BC, Ginsberg MH, Furie B. A platelet α-granule membrane protein that is associated with the plasma membrane after activation. J Clin Invest. 1986; 78:130-137. (Biology).
Bonfanti R, Furie BC, Furie B, Wagner DD. PADGEM (GMP-140) is a component of Weibel-Palade bodies of human endothelial cells. Blood. 1989; 73:1109-1112. (Biology).
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Diacovo T, Springer TA. CD62L (L-selectin) cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1503-1504.
Hamburger SA, McEver RP. GMP-140 mediates adhesion of stimulated platelets to neutrophils. Blood. 1990; 75:550-554. (Biology).
Larsen E, Celi A, Gilbert GE, et al. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes.. Cell. 1989; 59(2):305-12. (Biology). View Reference
Metzelaar MJ, Sixma JJ, Nieuwenhuis HK. Detection of platelet activation using activation-specific monoclonal antibodies. Blood Cells. 1990; 16:85-96. (Biology).
Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1038-1039.
Parmentier S, Kaplan C, Catimel B, McGregor JL. New families of adhesion molecules play a vital role in platelet functions.. Immunol Today. 1990; 11(7):225-7. (Biology). View Reference
Stenberg PE, McEver RP, Shuman MA, Jacques YV, Bainton DF. A platelet α-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation. J Cell Biol. 1985; 101:880-886. (Biology).

View All (12)

654976 Rev. 1

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