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CD1a PE
Product Details
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BD™
CD1; T6/Leu-6; R4; T6; HTA-1; FCB6
Human
Mouse IgG2b, κ
Human Thymocytes
Flow cytometry
25 μg/mL
20 μL
909
Phosphate buffered saline with gelatin and 0.1% sodium azide.
ASR


Preparation And Storage

Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

341641 Rev. 1
Antibody Details
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SK9

The CD1a antibody, clone SK9,1 is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from CD-1 mice immunized with human thymocytes.

The CD1a antibody recognizes a 49-kilodalton (kDa) type I transmembrane glycoprotein that associates non-covalently with β2-microglobulin.

341641 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
341641 Rev.1
Citations & References
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View product citations for antibody "341641" on CiteAb

Development References (8)

  1. Boumsell L, Amiot M, Raynal B, Gay-Bellile V, Caillou B, Bernard A. Epitope groups of CD1 molecules. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:289-302.
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
  4. Dalloul AH, Patry C, Salamero J, Canque B, Grassi F, Schmitt C. Functional and phenotypic analysis of thymic CD34+ CD1a- progenitor-derived dendritic cells: predominance of CD1a- differentiation pathway. J Immunol. 1999; 162:5821-5828. (Biology).
  5. Ito T, Inaba M, Inaba K, et al. A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.. J Immunol. 1999; 163(3):1409-19. (Biology). View Reference
  6. Martin LH, Calabi F, Lefebvre FA, Bilsland CA, Milstein C. Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.. Proc Natl Acad Sci USA. 1987; 84(24):9189-93. (Biology). View Reference
  7. Wood GS, Burns BF, Dorfman RF, Warnke RA. The immunohistology of non-T cells in the acquired immunodeficiency syndrome.. Am J Pathol. 1985; 120(3):371-9. (Biology). View Reference
  8. Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983; 131(1):212-216. (Biology). View Reference
View All (8) View Less
341641 Rev. 1

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