Description
Naturally occurring human CD4+ regulatory T cells (Tregs) are critical mediators of self-tolerance and suppress aberrant or inappropriate immune responses that can lead to conditions such as type 1 diabetes, rheumatoid arthritis, and graft-vs-host disease. The BD FastImmune™ kit measures expression of the activation markers CD154 and/or CD69 on effector T cells. Reduced expression of these markers in the presence of Tregs can indicate Treg suppressive capacity. The assay is performed in 96-well microplates using peripheral blood mononuclear cells (PBMCs) activated in the presence and absence of Tregs. It is a short-term assay that can be set up and analyzed in approximately one day. The T-cell markers, CD3 and CD4, are included in the kit for T-cell gating purposes, and CD25 is included for defining the Treg population.
Contents: Regulatory T-Cell function cocktail (CD3 PerCP-Cy™5.5, CD4 FITC, CD25 PE)| CD69 PE-Cy™7| CD154 APC| EDTA.
Preparation And Storage
Store reagents at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
Development References (8)
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Ashokkumar C, Talukdar A, Sun Q, et al. Allospecific CD154+ T cells associate with rejection risk after pediatric liver transplantation.. Am J Transplant. 2009; 9(1):179-91. (Biology). View Reference
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Baecher-Allan C, Brown JA, Freeman GJ, Hafler DA. CD4+CD25high regulatory cells in human peripheral blood. J Immunol. 2001; 167:1245-1253. (Biology).
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Bour-Jordan H, Bluestone JA. Regulating the regulators: costimulatory signals control the homeostasis and function of regulatory T cells.. Immunol Rev. 2009; 229(1):41-66. (Biology). View Reference
-
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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Chattopadhyay PK, Yu J, Roederer M. A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles.. Nat Med. 2005; 11(10):1113-7. (Biology). View Reference
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Clinical and Laboratory Standards Institute. 2005. (Biology).
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Disis ML, dela Rosa C, Goodell V, et al. Maximizing the retention of antigen specific lymphocyte function after cryopreservation. J Immunol Methods. 2006; 308:13-18. (Biology).
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Frentsch M, Arbach O, Kirchhoff D, et al. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression.. Nat Med. 2005; 11(10):1118-24. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.