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Purified Mouse Anti-SATB1
Purified Mouse Anti-SATB1

Western blot analysis of SATB1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1: 500, lane 3: 1: 1000 dilution of the mouse anti- SATB1 antibody.

Western blot analysis of SATB1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1: 500, lane 3: 1: 1000 dilution of the mouse anti- SATB1 antibody.

Product Details
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BD Transduction Laboratories™
SATB homeobox 1; Special AT-rich sequence-binding protein 1
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human SATB1 aa. 550-667
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
106 kDa
250 µg/ml
AB_398716
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611182 Rev. 2
Antibody Details
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14/SATB1

The 14/SATB1 monoclonal antibody specifically binds to the special AT-rich sequence binding protein 1 that is encoded by the SATB1 (SATB homeobox 1) gene. Central to the formation of nucleosomes, which consist of octameric histone cores with defined segments of chromatin wound around them, are the interactions of nucleosomes with nuclear matrix components. Specific genomic DNA segments that interact with the nuclear matrix are called scaffold or matrix attachment regions (SARs or MARs). SATB1 is a homeodomain MAR binding protein. It recognizes ATC sequences that consist of stretches of A's, T's, and C's on one DNA strand. SATB1 is expressed by thymocytes (especially single positive CD4+ thymocytes), T and B lymphocytes, NK cells, and macrophages. SATB1 can recruit chromatin-remodeling factors and thereby regulate chromatin structure and gene expression. Recently it has been reported that repression of SATB1 was essential for the phenotype and function of mouse regulatory T cells but inhibitory for the establishment of transcriptional programs expressed by effector T cells.

611182 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611182 Rev.2
Citations & References
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Development References (6)

  1. Alvarez JD, Yasui DH, Niida H, Joh T, Loh DY, Kohwi-Shigematsu T. The MAR-binding protein SATB1 orchestrates temporal and spatial expression of multiple genes during T-cell development. Genes Dev. 2000; 14(5):521-535. (Biology). View Reference
  2. Beyer M, Thabet Y, Müller R-U, et al. Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation. Nat Immunol. 2011; 12:898-907. (Clone-specific: Flow cytometry, Western blot). View Reference
  3. Cai S, Kohwi-Shigematsu T.. Intranuclear relocalization of matrix binding sites during T cell activation detected by amplified fluorescence in situ hybridization.. 1999; 19(3):394-402. (Biology). View Reference
  4. Dickinson LA, Joh T, Kohwi Y, Kohwi-Shigematsu T. A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition. Cell. 1992; 70(4):631-645. (Biology). View Reference
  5. Liu J, Bramblett D, Zhu Q. The matrix attachment region-binding protein SATB1 participates in negative regulation of tissue-specific gene expression. Mol Cell Biol. 1997; 17(9):5275-5287. (Biology). View Reference
  6. de Belle I, Cai S, Kohwi-Shigematsu T. The genomic sequences bound to special AT-rich sequence-binding protein 1 (SATB1) in vivo in Jurkat T cells are tightly associated with the nuclear matrix at the bases of the chromatin loops. J Cell Biol. 1998; 141(2):335-348. (Biology). View Reference
View All (6) View Less
611182 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.