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Purified Mouse Anti-Tom20
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Rat, Dog (Tested in Development)
Mouse IgG1
Human Tom20 aa. 47-145
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
18 kDa
250 µg/ml
AB_399595
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612278 Rev. 1
Antibody Details
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29/Tom20

Many mitochondrial proteins are encoded by nuclear genes and synthesized in the cytosol as pre-proteins. These pre-proteins are targeted to the mitochondria where they are translocated across the outer and inner membranes of the mitochondria. At the outer membrane of the mitochondria, this process requires a multiprotein complex called the Translocase of the Outer Membrane of the mitochondria (TOM). The TOM complex includes receptor components, Tom20, 22, 37, and 70, as well as a multisubunit general import pore complex of 400 kDa. Tom20 contains a hydrophobic N-terminal region that anchors it to the outer membrane of the mitochondria, and a cytosolic region that contains a domain that facilitates binding to the basic amphiphilic targeting sequences of pre-proteins and a tetratricopeptide repeat (TPR) motif involved in interactions with Tom70. In yeast, Tom20 and Tom22 mediate the import of all pre-proteins known to use the general import machinery of the mitochondria. Thus, Tom20 is an important receptor for many mitochondrial pre-proteins that use the outer mitochondrial translocation complex.

612278 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612278 Rev.1
Citations & References
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Development References (3)

  1. Abe Y, Shodai T, Muto T, et al. Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20. Cell. 2000; 100(5):551-560. (Biology). View Reference
  2. Hernandez JM, Giner P, Hernandez-Yago J. Gene structure of the human mitochondrial outer membrane receptor Tom20 and evolutionary study of its family of processed pseudogenes. Gene. 1999; 239(2):283-291. (Biology). View Reference
  3. Krimmer T, Rapaport D, Ryan MT, et al. Biogenesis of porin of the outer mitochondrial membrane involves an import pathway via receptors and the general import pore of the TOM complex. J Cell Biol. 2001; 152(2):289-300. (Biology). View Reference
612278 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.