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Western blot analysis of Gephyrin on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Gephyrin.
Rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment, 20X.
BD Transduction Laboratories™ Purified Mouse Anti-Gephyrin
BD Transduction Laboratories™ Purified Mouse Anti-Gephyrin
Purified Mouse Anti-Gephyrin
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The sub-membraneous region of the postsynaptic neuron is an intricate network of cytoskeletal elements generally known as the postsynaptic density. It is thought that this elaborate cytoskeletal region is critical for receptor targeting, clustering, and efficient signal input. Gephyrin, a 93 kDa protein, was identified as a result of its ability to bind to polymerized tubulin (microtubules). Although expressed in all tissues, gephyrin mRNA is found most abundantly in brain. Alternative splicing produces at least five different mRNAs. Gephyrin colocalizes and interacts with the glycine receptor at the postsynaptic density. It is possible that Gephyrin acts as an anchor between the glycine receptor and microtubules.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (4)
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Feng G, Tintrup H, Kirsch J, et al. Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity. Science. 1998; 282(5392):1321-1324. (Clone-specific: Western blot). View Reference
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Kneussel M, Haverkamp S, Fuhrmann JC, et al. The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse. Proc Natl Acad Sci U S A. 2000; 97(15):8594-8599. (Clone-specific: Immunofluorescence, Western blot). View Reference
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Meyer G, Kirsch J, Betz H, Langosch D. Identification of a gephyrin binding motif on the glycine receptor beta subunit. Neuron. 1995; 15(3):563-572. (Biology). View Reference
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Prior P, Schmitt B, Grenningloh G, et al. Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein. Neuron. 1992; 8(6):1161-1170. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.