Preparation And Storage
Recommended Assay Procedures
ELISA: Purified 5A1 anti-mouse CSF-1 antibody (Cat. No. 552513) is useful as a capture antibody for a sandwich ELISA which measures mouse CSF-1 protein levels. Purified 5A1 anti-mouse CSF-1 antibody can be paired with a biotinylated antibody (such as D24) as the detector, with recombinant mouse CSF-1 protein as the standard. This capture antibody should be titrated between 1 -4 µg/ml to determine the optimal coating concentration for ELISA. To obtain linear standard curves, doubling dilutions of recombinant mouse CSF-1 ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines:
IL-1RI, IL-1RII, IL-1α, IL-1β, IL-2, IL-2sR-α, IL-3, IL-4, IL-5, IL-6, IL-6R gp130, IL-7, IL-8, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-15, TNF, LT-α, sTNFRI, sTNFRII, GM-CSF, TGFβ, IFNγ, CD40L, TRAIL, FasL.
Neutralization: Other reported applications include in vitro neutralization of CSF-1 bioactivity. The no azide/low endotoxin (NA/LE) format of 5A1 (Cat. No. 557858) is recommended for in vitro assays.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The macrophage-specific CSF (known as M-CSF or CSF-1) is hematopoietic growth factor that regulates the survival, proliferation, differentiation of mononuclear phagocytes. CSF-1 are found to be produced by a vast number of cells, and including fibroblasts, stromal cells, osteoblasts, and activated T cells, macrophages, and B cells.
This antibody is routinely tested by ELISA capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (2)
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Lokeshwar BL, Lin HS. A sandwich enzyme-linked immunoadsorbent assay for detection of murine macrophage colony-stimulating factor (CSF-1). J Immunol Methods. 1989; 123(1):123-129. (Clone-specific: ELISA). View Reference
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Lokeshwar BL, Lin HS. Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor. J Immunol. 1988; 15(2):483-488. (Clone-specific: ELISA). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
