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Purified NA/LE Rat Anti-Mouse IL-12 (p40/p70)
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat IgG2a
Recombinant Mouse IL-12 p70
ELISA (Routinely Tested), Neutralization (Tested During Development), Immunoprecipitation, Western blot (Reported)
1.0 mg/ml
AB_395418
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Assay Procedures

ELISA (IL-12 p70 Detection):  Biotin Rat Anti-Mouse IL-12 (p40/p70) antibody (Clone C17.8, Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA for specifically measuring mouse IL-12 p70 protein levels. Biotinylated C17.8 antibody can be paired with the Purified Rat Anti-Mouse IL-12 p70 antibody (Clone 9A5, Cat. No. 554658) as the capture antibody and with recombinant mouse IL-12 p70 (Cat. No.554592) as the standard.  For measuring mouse IL-12 p70 in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-12 p70 ELISA Set (Cat. No. 555256).

ELISA (IL-12 p40 Detection):  Biotin Rat Anti-Mouse IL-12 (p40/p70) antibody (Clone C17.8, Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA measuring IL-12 p40 protein levels (free p40 monomer as well as p40 complexes as homodimer or heterodimer). Biotinylated C17.8 antibody can be paired with the Purified Rat Anti-Mouse IL-12 p40/p70 antibody (Clone C15.6, Cat. No. 551219) as the capture antibody, and with recombinant mouse IL-12 p70 protein (Cat. No.554592) or IL-12 p40 protein (Cat. No. 554594) as the standard.  For measuring mouse IL-12 p40 in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-12 p40 ELISA Set (Cat. No. 555165).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
554475 Rev. 3
Antibody Details
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C17.8

The C17.8 antibody reacts with the free p40 monomer of IL-12 as well as the p40 subunit complexed as homodimer or complexed with p35 as a p70 heterodimer. Clone C17.8 has also been shown to be cross-reactive with mouse IL-23, which also contains the p40 subunit. The immunogen used to generate C17.8 hybridoma was recombinant mouse IL-12 p70.

554475 Rev. 3
Format Details
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NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
554475 Rev.3
Citations & References
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Development References (4)

  1. D'Andrea A, Rengaraju M, Valiante NM, et al. Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J Exp Med. 1992; 176(5):1387-1398. (Biology). View Reference
  2. Maruo S, Ahn HJ, Yu WG, et al. Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity. J Leukoc Biol. 1997; 61(3):346-352. (Biology). View Reference
  3. Neurath MF, Fuss I, Kelsall BL, Stuber E, Strober W. Antibodies to interleukin 12 abrogate established experimental colitis in mice. J Exp Med. 1995; 182(5):1281-1290. (Biology). View Reference
  4. Wysocka M, Kubin M, Vieira LQ, et al. Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice. Eur J Immunol. 1995; 25(3):672-676. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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554475 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.