Preparation And Storage
Recommended Assay Procedures
ELISA Capture: The purified 359-238-8 antibody (Cat. No. 551222) is useful as a capture antibody for a sandwich ELISA for measuring human LT-α protein levels. Purified 359-238-8 antibody can be paired with the biotinylated 359-81-11 antibody (Cat. No. 554555) as the detection antibody, with recombinant human LT-α (Cat. No. 554619) as the standard. Purified 359-238-8 antibody should be titrated 2 - 6 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of human LT-α ranging from ~2,000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, see the Immune Function Handbook also posted on the website at www.bdbiosciences.com
Note: This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Note: This ELISA pair is recommended for assay of suparnatant samples from experimental cell culture systems. For detection of human Lt-α in serum or plasma, the BD OptEIA™ human Lt-α ELISA Set, Cat. No. 550995 is specially formulated and recommended.
WB: The 359-238-8 antibody is not recommended for Western blotting.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The 359-238-8 antibody reacts with human lymphotoxin-α (LT-α, formerly known as TNF-β). The immunogen used to generate the 359-238-8 hybridoma was recombinant human LT-α. This is a neutralizing antibody.
Development References (2)
-
Meager A, Parti S, Leung H, Peil E, Mahon B. Preparation and characterization of monoclonal antibodies directed against antigenic determinants of recombinant human tumour necrosis factor (rTNF). Hybridoma. 1987; 6(3):305-312. (Clone-specific: ELISA, Neutralization). View Reference
-
Meager A, Parti S, Leung H, et al. A two-site sandwich immunoradiometric assay of human lymphotoxin with monoclonal antibodies and its applications. J Immunol Methods. 1987; 104(2):31-42. (Clone-specific: ELISA, Neutralization). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
