BD Pharmingen™ Purified Mouse Anti-Armenian and Syrian Hamster IgG

This antibody has been tested by ELISA to assure specificity and reactivity. For the sandwich Biotin Mouse Anti-Armenian and Syrian Hamster IgG (Cat. No. 554025) is optimal for detection with Purified Mouse Anti-Armenian Hamster IgG2 (Cat. No. 550638) for capture.

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.