ELISA Detection: Biotin Rat Anti-Mouse IL-6 antibody (Cat. No. 554402) is useful as a detection antibody for a sandwich ELISA for measuring mouse IL-6 protein levels. Biotin Rat Anti-Mouse IL-6 antibody can be paired with the Purified Rat Anti-Mouse IL-6 antibody (Cat. No. 554400) as the capture antibody, with Recombinant Mouse IL-6 (Cat. No. 554582) as the standard. Biotin Rat Anti-Mouse IL-6 antibody should be pre-titered in the range of 0.5 to 2 µg/ml to determine its optimal concentration for ELISA. To obtain linear standard curves, doubling dilutions of mouse IL-6 ranging from ~2,000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our website, http://www.bdbiosciences.com/us/s/resources, and go to the protocols section under "ELISA and ELISPOT".
Note 1: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring mouse IL-6 in serum or plasma our Mouse IL-6 ELISA Set (Cat. No. 555240) or Mouse IL-6 ELISA Kit (Cat. No. 550950) are specially formulated and recommended.
Note 2: This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Western blot: Biotin Rat Anti-Mouse IL-6 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen.