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Mouse IL-23 p19/p40 Flex Set

Mouse IL-23 p19/p40 Flex Set

(RUO)
Mouse IL-23 p19/p40 Flex Set

Figure 1. Example BD CBA Mouse IL-23 p19/p40 Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Mouse IL-23 p19/p40 Flex Set

Linearity: Cell culture supernatant was spiked with recombinant mouse IL-23 p19/p40 protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Mouse IL-23 p19/p40 Flex Set

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse IL-23 p19/p40 Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).

Mouse IL-23 p19/p40 Flex Set

Recovery: Cell culture supernatant was spiked with three different levels of recombinant mouse IL-23 p19/p40 protein. The spiked samples were assayed and the results were compared with expected values.

Figure 1. Example BD CBA Mouse IL-23 p19/p40 Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Linearity: Cell culture supernatant was spiked with recombinant mouse IL-23 p19/p40 protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse IL-23 p19/p40 Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).

Recovery: Cell culture supernatant was spiked with three different levels of recombinant mouse IL-23 p19/p40 protein. The spiked samples were assayed and the results were compared with expected values.

Product Details
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BD™ Cytometric Bead Array (CBA)
D7
10-2,500 pg/mL
Mouse (QC Testing)
RUO
AB_2869425


Description

The BD™ CBA Mouse IL-23 (p19/p40) Flex Set is a bead-based immunoassay capable of measuring mouse Interleukin-23 (p19/p40) in cell culture supernatant samples. Investigators are encouraged to titrate cell culture supernatant samples, such as with a 1:2 dilution with CBA Assay Diluent, prior to testing. The BD™ CBA Mouse IL-23 (p19/p40) Flex Set is not recommended for testing mouse serum or plasma samples. Mouse reactivity was determined by testing samples with the BD CBA Mouse IL-23 (p19/p40) Flex Set. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266 or 558267).

Preparation And Storage

This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.

Recommended Assay Procedures

The BD CBA Mouse IL-23 (p19/p40) Flex Set must be used in conjunction with a BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266, 100 tests, or 558267, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions for the use of this product can be found in the manual for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Mouse IL-23 (p19/p40) Flex Set will be 2,500 pg/mL.

The BD CBA Mouse IL-23 (p19/p40) Flex Set should not be used in the same assay well with any non-BD CBA Mouse Soluble Protein Flex Set Reagents, which include: Human Soluble Protein, Human Enhanced Sensitivity, Mouse Enhanced Sensitivity, Human Soluble Receptor and Cell Signalling Flex Sets.  For an updated assay compatibility chart for the BD CBA Mouse Soluble Protein Flex Sets, please visit http://www.bdbiosciences.com/cbasetup.

Performance

Limit of Detection: The theoretical limit of detection is 1.04 pg/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/mL, n=30) + 2 standard deviations.

Product Notices

  1. ProClin is a trademark of Rohm and Haas Company.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
562575 Rev. 4
Components
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Description Quantity/Size Part Number
Mouse IL-23 p19/p40 Standard N/A 51-9008106
Mouse IL-23 p19/p40 PE Detection Reagent N/A 51-9008108
Mouse IL-23 p19/p40 Capture Bead D7 100 Tests (1 ea) 51-9008110
562575 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.