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      Human Enhanced Sensitivity Master Buffer Kit

      BD™ Cytometric Bead Array (CBA) Human Enhanced Sensitivity Master Buffer Kit

      (RUO)
      Product Details
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      BD™ Cytometric Bead Array (CBA)
      Human (QC Testing)
      Flow cytometry (Routinely Tested)
      RUO


      Description

      The BD CBA Human Enhanced Sensitivity Master Buffer Kit contains all of the supporting reagents necessary to perform an assay using a BD CBA Human Enhanced Sensitivity Flex Set. The buffers, Enhanced Sensitivity Detection Reagent (Part B), and instrument setup reagents provided in this kit have been optimized for analysis of analytes in tissue culture supernatants, plasma, and serum samples. The BD CBA Human Enhanced Sensitivity Master Buffer Kit provides sufficient reagents for the quantitative analysis of 100 samples and 10 instrument setup procedures.

      Preparation And Storage

      Store all kit components at 2 to 8°C. Do not freeze.

      Investigators are advised that Assay Diluent can exhibit color variation. All components of this kit, except for the Enhanced Sensitivity Detection Reagent (Part B), contain sodium azide. Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

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      Recommended Assay Procedures

      The Human Enhanced Sensitivity Master Buffer Kit has been optimized for use with the BD CBA Human Enhanced Sensitivity Flex Sets and should not be used with Flex Sets from any other BD CBA System (soluble protein, cell signalling, etc). For an assay compatibility chart for the BD CBA Human Enhanced Sensitivity Flex Sets, please visit bdbiosciences.com/cbasetup.

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      561521 Rev. 2
      Components
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      Description Quantity/Size Part Number EntrezGene ID
      Assay Diluent N/A 51-9003988 N/A
      Capture Bead Diluent 100 Tests (1 ea) 51-9003803 N/A
      Detection Reagent Diluent 100 Tests (1 ea) 51-9007527 N/A
      Wash Buffer N/A 51-9007221 N/A
      Instrument Setup Bead A1 N/A 51-9003855 N/A
      Instrument Setup Bead A9 N/A 51-9003858 N/A
      Instrument Setup Bead F1 100 Tests (1 ea) 51-9003851 N/A
      Instrument Setup Bead F9 N/A 51-9003854 N/A
      PE Instrument Setup Bead F1 100 Tests (1 ea) 51-9005038 N/A
      PE* Positive Control Detector 100 Tests (1 ea) 51-9005065 N/A
      Enhanced Sensitivity Detection Reagent N/A 51-9007218 N/A
      561521 Rev. 2
      Citations & References
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      Development References (10)

      1. Bishop JE, Davis KA. A flow cytometric immunoassay for β2-microglobulin in whole blood. J Immunol Methods. 1997; 210:79–87. (Biology).
      2. Camilla C, Defoort JP, Delaage M, et al. A new flow cytometry-based multi-assay system 1, Application to cytokine immunoassays. Cytometry Suppl. 1998; 8:132. (Biology).
      3. Carson R, Vignali D. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999; 227:41–52. (Biology).
      4. Chen, R, L Lowe, et al. Simultaneous quantification of six human cytokines in a single sample using microparticle-based flow cytometric technology. Clin Chem. 1999; 9:1693-1694. (Biology).
      5. Cook, EB, JL Stahl, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. J Immunol Methods. 2001; 254:109-118. (Biology).
      6. Dotti, G, B Salvodo, et al. Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells: A model for immunotherapy. Exp Hematol. 2001; 29:952-961. (Biology).
      7. Fulwyler M, McHugh TM. Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes. Methods Cell Biol. 1994; 33:613-629. (Biology).
      8. Kricka LJ. Diamandis EP, Christopoulos TK, ed. Immunoassay. Academic Press; 1996:389-404.
      9. Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Biology). View Reference
      10. Oliver KG, Kettman JR, Fulton RJ. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Clin Chem. 1998; 44:2057-2060. (Biology).
      View All (10) View Less
      561521 Rev. 2

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.