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      Purified NA/LE Mouse Anti-Human CD28
      567117
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      EA (1 Each)
      2.0 mg
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      Product Details
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      BD Pharmingen™
      CD28 antigen; T44; Tp44; TP44
      Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
      Mouse C3H x BALB/c IgG1, κ
      Human CD28 Transfected Cell Line
      Flow cytometry (Routinely Tested), Activation (Tested During Development)
      1.0 mg/ml
      V 5T CD28.05
      940
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, sterile filtered(0.2µm pore size membrane). Endotoxin level is ≤0.1 EU/µg (≤0.01 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Recommended Assay Procedures

      The CD28.2 monoclonal antibody is reportedly useful for multiple applications including the co-stimulated increase in cytokine production and proliferative responses by Anti-CD3 antibody-stimulated T cells.

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      Data Sheets

      DownloadTechnical Data Sheet (TDS)DownloadSafety Data Sheet
      567117 Rev. 1
      Antibody Details
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      CD28.2

      The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells.

              

      567117 Rev. 1
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is =0.01 EU/µg (=0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      567117 Rev.1
      Citations & References
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      No Citations Found

      No Citations Are Available for this Product

      567117 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.