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Human BD Fc Block™
Human BD Fc Block™

Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with BD Pharmingen™ Human BD Fc Block™. Human peripheral blood mononuclear cells were either not treated (dashed line histogram) or preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/ 564220; solid line histogram).  The cells were then stained with an excess of irrelevant PE-conjugated Mouse IgG2a antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with BD Pharmingen™ Human BD Fc Block™. Human peripheral blood mononuclear cells were either not treated (dashed line histogram) or preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/ 564220; solid line histogram).  The cells were then stained with an excess of irrelevant PE-conjugated Mouse IgG2a antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Product Details
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BD Pharmingen™
Human (QC Testing), Rhesus (Tested in Development)
Human IgG1
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_2728082
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Recommended Assay Procedures

Incubate 1 million cells suspended in 50-100 µL of staining buffer with 2.5 µg of Human BD Fc Block™ (10 minutes at room temperature) followed by staining with the desired fluorescent antibody. No washing step is needed between the blocking and staining steps.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
564219 Rev. 3
Antibody Details
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Fc1.3216

Fcγ Receptors belong to the immunoglobulin superfamily and are expressed at varying levels in multiple cell lineages including high expression in myeloid and B cells. The major functions of Fc receptors are protective functions of the immune system. There are multiple different types of Fc receptors reflecting a variety of different biological activities, which are modulated when they are aggregated by multivalent antigen-antibody complexes.

While normally serving important physiological roles in the immune system, Fc Receptors can also be the cause of nonspecific, false-positive antibody staining of cells. Human BD Fc Block™ is designed and formulated to block or significantly reduce potential non-specific antibody staining caused by receptors for IgG that may be encountered in various applications including the flow cytometric analysis of human cells.  Moreover, it can increase the specificity of antibody labeling of extremely rare target cells such as antigen-specific B cells, fetal cells in maternal blood, hematopoietic progenitor cells, or disseminated epithelial tumor cells.

564219 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
564219 Rev.3
Citations & References
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Development References (2)

  1. Nimmerjahn F, Ravetch JV. Fc-receptors as regulators of immunity. Adv Immunol. 2007; 96:179-204. (Biology). View Reference
  2. Ravetch JV, Bolland S. IgG Fc receptors. Annu Rev Immunol. 2001; 19:275-290. (Biology). View Reference
564219 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.