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APC Mouse Anti-Human CD200
APC Mouse Anti-Human CD200
Multiparameter flow cytometric analysis of CD200 expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human CD3 antibody (Cat. No. 555332/561806/561807) and either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; Left Plots) or APC Mouse Anti-Human CD200 antibody (Cat. No. 566893/566894; Right Plots). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Bivariate pseudocolor density plots showing the correlated expression of CD200 (or Ig Isotype control staining) versus side-light scatter (SSC-A) signals (Top Plots) or CD3 (Bottom Plots) were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations or lymphocytes, respectively. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Multiparameter flow cytometric analysis of CD200 expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human CD3 antibody (Cat. No. 555332/561806/561807) and either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; Left Plots) or APC Mouse Anti-Human CD200 antibody (Cat. No. 566893/566894; Right Plots). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Bivariate pseudocolor density plots showing the correlated expression of CD200 (or Ig Isotype control staining) versus side-light scatter (SSC-A) signals (Top Plots) or CD3 (Bottom Plots) were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations or lymphocytes, respectively. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Product Details
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BD Pharmingen™
OX-2; MOX1; MOX2 ; My033
Human (QC Testing)
Mouse IgG1, κ
Human CD200 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
VII 70655; IX 40
4345
AB_2869940
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566893 Rev. 1
Antibody Details
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MRC OX-104

The MRC OX-104 monoclonal antibody specifically binds to CD200. CD200 is a 40-45 kDa type 1 transmembrane glycoprotein and is also referred to as OX2. CD200 is a member of the immunoglobulin superfamily of proteins. It contains two Ig domains, a single transmembrane region and a short cytoplasmic tail. CD200 is expressed on some dendritic cell subsets, and resting and activated T- and B-cells, but not on NK cells, monocytes, granulocytes, or platelets. It has been found on a subset of CD34+ progenitor cells. Interaction of CD200 with its receptor on macrophages induces a downregulation of macrophage activity in a variety of tissues, suggesting a regulatory function.

        

566893 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
566893 Rev.1
Citations & References
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View product citations for antibody "566893" on CiteAb

Development References (4)

  1. Barclay AN, Wright GJ, Brown MH. CD200 (OX2) Summary and Workshop report: The lymphoid/ neuronal OX2 glycoprotein (CD200) interacts with a novel receptor on macrophages. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:471-473.
  2. Hoek RM, Ruuls SR, Murphy CA, et al. Down-regulation of the macrophage lineage through interaction with OX2 (CD200).. Science. 2000; 290(5497):1768-71. (Biology). View Reference
  3. Wright GJ, Jones M, Puklavec MJ, Brown MH, Barclay AN. The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans. Immunology. 2001; 102(2):173-179. (Immunogen: ELISA, Flow cytometry, Immunohistochemistry). View Reference
  4. Wright GJ, Puklavec MJ, Willis AC, et al. Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function. Immunity. 2000; 13(2):233-242. (Biology). View Reference
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566893 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.