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      Alexa Fluor® 647 Mouse anti-Human CD337 (NKp30)
      Alexa Fluor® 647 Mouse anti-Human CD337 (NKp30)
      Expression of CD337 (NKp30) in human NK cells.  Human blood cells were stained with either Alexa Fluor® 647 Mouse anti-Human CD337 (NKp30) (Cat. No. 558408; right panel) or Alexa 647 mouse IgG1 isotype control (Cat. No. 557714; left panel) and Alexa Fluor 488 Mouse anti-Human CD56 (Cat. No. 557699).  Erythrocytes were lysed using BD PharmLyse™ lysis buffer (Cat. No. 555899).  Viable lymphocytes were selected by scatter profile.  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      Alexa Fluor® 647 Mouse anti-Human CD337 (NKp30)
      Expression of CD337 (NKp30) in human NK cells.  Human blood cells were stained with either Alexa Fluor® 647 Mouse anti-Human CD337 (NKp30) (Cat. No. 558408; right panel) or Alexa 647 mouse IgG1 isotype control (Cat. No. 557714; left panel) and Alexa Fluor 488 Mouse anti-Human CD56 (Cat. No. 557699).  Erythrocytes were lysed using BD PharmLyse™ lysis buffer (Cat. No. 555899).  Viable lymphocytes were selected by scatter profile.  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      Product Details
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      BD Pharmingen™
      NCR3; Natural cytotoxicity triggering receptor 3; NKp30; MALS; LY117; 1C7
      Human (QC Testing)
      Mouse IgG1, κ
      Horse NKp30 extracellular domain
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      259197
      AB_647154
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      558408 Rev. 2
      Antibody Details
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      p30-15

      The p30-15 monoclonal antibody specifically binds to CD337, also known as NKp30, a receptor found on the surface of natural killer (NK) cells.  NK cells are large lymphoid cells discovered because of their ability to recognize and kill abnormal cells such as tumor and virally infected cells.  NK cell immune responses are regulated by a balance of activating and inhibitory signals generated by cell surface receptors.  Inhibitory receptors recognize MHC class I molecules on normal cells producing a negative signal to the NK cell.  Loss of MHC class I expression in infected or transformed cells results in the loss of this negative signal leading to NK cell activation.  In concert with the loss of inhibitory signals, activation signals via NK receptors such as NKp30, NKp44, NKp46, NKG2D, and NKp80 mediate the activation of NK cells.  NKp30 cooperates with NKp46 and/or NKp44 in the induction of NK cell-mediated cytotoxicity against the majority of target cells.

      558408 Rev. 2
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      558408 Rev.2
      Citations & References
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      View product citations for antibody "558408" on CiteAb

      Development References (5)

      1. Augugliaro R, Parolini S, Castriconi , et al. Selective cross-talk among natural cytotoxicity receptors in human natural killer cells. Eur J Immunol. 2003; 33(5):1235-1241. (Biology). View Reference
      2. Byrd A, Hoffmann SC, Jarahian M, Momburg F, Watzl C. Expression analysis of the ligands for the Natural Killer cell receptors NKp30 and NKp44. PLoS ONE. 2007; 2(12):e1339. (Immunogen). View Reference
      3. Flaig RM, Stark S, Watzl C. Cutting edge: NTB-A activates NK cells via homophilic interaction. J Immunol. 2004; 172(11):6524-6527. (Biology). View Reference
      4. Pende D, Parolini S, Pessino A, et al. Identification and molecular characterization of NKp30, a novel triggering receptor involved in natural cytotoxicity mediated by human natural killer cells. J Exp Med. 1999; 190(10):1505-1516. (Biology). View Reference
      5. Stark S, Flaig RM, Sandusky M, Watzl C. The use of trimeric isoleucine-zipper fusion proteins to study surface-receptor-ligand interactions in natural killer cells. J Immunol Methods. 2004; 296(1-2):149-158. (Biology). View Reference
      View All (5) View Less
      558408 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.