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IgM-PerCP-Cyanine5.5
Product Details
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BD Cytognos™
IgM
Human
Mouse IgG1
Human IgM+ memory B cells and plasma cells
3 µl/test
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


cyt-igmc2 Rev. 1
Antibody Details
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MHM-88

IgM antibody, clone MHM-88, is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgM expressing cells. Monomeric IgM is always the first form and isotype of Ig generated by naive B cells. Following its initial activation by antigen, the naive B cell proliferates and differentiates, and its progeny produce the pentameric secreted form of IgM. Thus, it is IgM antibodies that are expressed first in any primary immune response.

cyt-igmc2 Rev. 1
Format Details
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PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
cyt-igmc2 Rev.1
Citations & References
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cyt-igmc2 Rev. 1

 

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.