Description
For use in the setup and verification of the doublet discrimination function of FACSCalibur™, FACSort™, or FACScan™ flow cytometers equipped with a doublet discrimination module (DDM) for DNA analysis, and for monitoring daily instrument performance. For use with other FACS® brand flow cytometers, all performance specifications will need to be determined for each individual case although the particles will meet the stability criteria as stated.
Contents: Chicken erythrocyte nuclei (CEN)| calf thymocyte nuclei (CTN)| instrument QC beads| propidium iodide solution.
Preparation And Storage
1. When stored at 2° to 8°C, the reagent is stable until the expiration date shown on the label. Do not use after the expiration date. Keep the reagent vials dry.
2. There is a tendency for the CEN (vial A) to settle during storage. This can be corrected by gently vortexing before use. Vortexing too vigorously will separate desired aggregates. It is critical that CEN be stored at 2° to 8°C, since these particles are sensitive to thermal degradation.
3. CTN (vial B) tend to aggregate during storage and should be vigorously vortexed to separate these aggregates.
4. The 2-µm beads (vial C) must be protected from prolonged exposure to light.
5. Do not expose stained CEN or CTN to prolonged light during storage or incubation.
6. Incubation times or temperatures other than those specified may lead to erroneous results.
7. Propidium iodide solution (vial D) must be protected from prolonged exposure to light. Deterioration in stain performance has been observed with short periods of room temperature storage, therefore stained samples must be stored at 2° to 8°C in the dark until analysis (maximum 12 hours).
Development References (7)
-
Barlogie B, et al. Flow cytometry in clinical cancer research. Cytometry. 1983; 43:3982-3997. (Biology).
-
Frankfurt OS, et al. Proliferative characteristics of primary and metastatic human solid tumors by DNA flow cytometry. Cytometry. 1984; 5:629-635. (Biology).
-
Hiddemann W, Schumann J, Andreef M, et al. Convention on nomenclature for DNA cytometry. Cytometry. 1984; 5:445-446. (Biology).
-
Kute TE, et al. Flow cytometry in solid-tumor prognosis. Lab Management. 1987; July:21-32. (Biology).
-
Larsen JK, et al. Flow cytometric discrimination of mitotic cells: resolution of M, as well as G1, S, and G2 phase nuclei with mithramycin, propidium iodide, and ethidium bromide after fixation with formaldehyde. Cytometry. 1985; 7:54-63. (Biology).
-
Ronot X, et al. G2 Arrest, binucleation, and single parameter DNA flow cytometric analysis. Cytometry. 1986; 7:286-290. (Biology).
-
Rose NR, et al. Manual of Clinical Laboratory Immunology 3rd ed. Washington, DC: American Society for Microbiology; 1986:234.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
