CD79a PerCP-Cy™5.5

BD™ CD79a PerCP-Cy™5.5

Name: CD79a PerCP-Cy™5.5
Brand: BD™
SKU: 341633

Clone HM47

(ASR)

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Product Details

Brand: BD™

Alternative Name: CD79a, immunoglobulin-associated alpha ; CD79A; Ig-alpha; IGA; Igα; MB-1

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human CD79a Peptide

Application: Flow cytometry

Concentration: 3.0 μg/mL

Vol. Per Test: 20 μL

Workshop Number: V cB017

Entrez Gene ID: 973

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: ASR

Preparation And Storage

Store vials at 2°C to 8°C and protect from light. Do not freeze. The reagent is stable until the date shown on the vial label when stored as directed.

341633 Rev. 1

Antibody Details

HM47

The CD79a antibody, clone HM47, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with a synthetic peptide conjugated to thyroglobulin. The peptide has the sequence representing amino acids 202–216 inclusive as deduced from the human mb-1 cDNA sequence.

The CD79a antibody recognizes the cytoplasmic domain of a 47-kilodalton (kDa) membrane glycoprotein present on B lymphocytes.

341633 Rev. 1

Format Details

PerCP-Cy5.5

PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PerCP-Cy5.5

Excitation Source: Blue 488 nm

Excitation Max: 482 nm

Emission Max: 676 nm

341633 Rev.1

Citations & References

View product citations for antibody "341633" on CiteAb

Development References (5)

Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Engel P, Wagner N, Tedder TF. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:667-679.
Mason DY, Cordell JL, Tse AG, et al. The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991; 147(11):2474-2482. (Biology). View Reference
Sakaguchi N, Kashiwamura S, Kimoto M, Thalmann P, Melchers F. B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties. EMBO J. 1988; 7(11):3457-3464. (Biology). View Reference

View All (5)

341633 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

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