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CD49d APC-H7
Product Details
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BD™
Integrin α4 chain; Integrin alpha 4; ITGA4; IA4; alpha 4 subunit of VLA-4
Human
Mouse IgG1, κ
Flow cytometry
25 μg/mL
5 μL
V S215
3676
ASR


656153 Rev. 1
Antibody Details
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9F10

The CD49d antibody, clone 9F10, is derived from the hybridization of mouse myeloma cells with spleen cells isolated from immunized mice.

The CD49d antibody recognizes the 150-kilodalton (kDa) type I transmembrane glycoprotein, integrin α. The CD49d antigen associates with one of two β integrin subunits, β(CD29) or β, to form the integrins αβ(VLA-4) or αβ, respectively.

656153 Rev. 1
Format Details
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APC-H7
The BD Horizon™ APC-H7 dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 659 nm and an acceptor dye, H7, with an emission maximum (Em Max) at 782 nm. APC-H7, driven by BD innovation, is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 780 nm (e.g., a 760/60 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC-H7
Red 627-640 nm
659 nm
782 nm
656153 Rev.1
Citations & References
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View product citations for antibody "656153" on CiteAb

Development References (13)

  1. Albelda SM, Buck CA. Integrins and other cell adhesion molecules.. FASEB J. 1990; 4(11):2868-80. (Biology). View Reference
  2. Barclay AN, Brown MH, Law SKA, McKnight AJ, Tomlinson MG, van der Merwe PA. The Leucocyte Antigen FactsBook 2nd ed. San Diego: Academic Press; 1997.
  3. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. Dittel BN, McCarthy JB, Wayner EA, LeBien TW. Regulation of human B-cell precursor adhesion to bone marrow stromal cells by cytokines that exert opposing effects on the expression of vascular cell adhesion molecule-1 (VCAM-1).. Blood. 1993; 81(9):2272-82. (Biology). View Reference
  6. Elices MJ, Osborn L, Takada Y, et al. VCAM-1 on activated endothelium interacts with the leukocyte integrin VLA-4 at a site distinct from the VLA-4/fibronectin binding site.. Cell. 1990; 60(4):577-84. (Biology). View Reference
  7. Hemler ME, Kassner P, Bodorova J. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:1617-1618.
  8. Lobb RR, Hemler ME. The pathophysiologic role of α 4 integrins in vivo. J Clin Invest. 1994; 94:1722-1728. (Biology).
  9. Nojima Y, Humphries MJ, Mould AP, et al. VLA-4 mediates CD3-dependent CD4+ T cell activation via the CS1 alternatively spliced domain of fibronectin.. J Exp Med. 1990; 172(4):1185-92. (Biology). View Reference
  10. Shimizu Y, Newman W, Gopal TV, et al. Four molecular pathways of T cell adhesion to endothelial cells: roles of LFA-1, VCAM-1, and ELAM-1 and changes in pathway hierarchy under different activation conditions.. J Cell Biol. 1991; 113(5):1203-12. (Biology). View Reference
  11. Shimizu Y, van Seventer GA, Horgan KJ, Shaw S. Costimulation of proliferative responses of resting CD4+ T cells by the interaction of VLA-4 and VLA-5 with fibronectin or VLA-6 with laminin.. J Immunol. 1990; 145(1):59-67. (Biology). View Reference
  12. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). View Reference
  13. Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
View All (13) View Less
656153 Rev. 1

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Analyte Specific Reagent. Analytical and performance characteristics are not established.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

 

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