Skip to main content Skip to navigation


A Strategy for Single-Cell Metabolic Analysis


A complex interaction of anabolic and catabolic metabolism underpins the ability of leukocytes to mount an immune response. Their capacity to respond to changing environments by metabolic reprogramming is crucial to effector function. However, current methods lack the ability to interrogate this network of metabolic pathways at the single-cell level within a heterogeneous population. Using Met-Flow, a flow cytometry–based method, the metabolic state of immune cells can be captured by targeting key proteins and rate-limiting enzymes across multiple pathways



New technologies for measuring divergent metabolic profiles


New technologies including antibody panels, flow cytometers and advanced data-analysis tools have been developed to simultaneously measure divergent metabolic profiles and dynamic remodeling in human peripheral blood mononuclear cells.



BD Biosciences hosted a webinar with Dr. John Connolly, a world-renowned immunologist and flow cytometry expert


Key topics covered in this webinar include:

  • How staining and analysis of metabolic and cell surface proteins can be made simultaneously at the single-cell level
  • How a novel high-parameter flow cytometry method utilizing antibodies against metabolic proteins that are critical and rate-limiting in their representative pathways can shed light onto the metabolic state of any given cell type
  • How various advanced data-analysis tools can be utilized to derive unique correlations between immune subpopulations and specific metabolic pathways


View the webinar >

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.