FITC Mouse Anti-Rat CD3
Clone G4.18 (RUO)
- Brand BD Pharmingen™
- Alternative Name CD3 Complex; T3
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG3, κ
- Reactivity Rat (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen PHA-stimulated rat lymph node and spleen cells
- Storage Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The G4.18 monoclonal antibody specifically recognizes the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4, blocks staining with mAb G4.18.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.