Purified Rat Anti-Human CD104
Clone 439-9B (RUO)
- Brand BD Pharmingen™
- Alternative Name Integrin β4 chain; Integrin beta 4; ITGB4; GP150; TSP-180
- Concentration 0.5 mg/ml
- Isotype Rat F344, also known as Fischer, CDF IgG2b, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human CD104 Protein
- Workshop No. V BP480, S247
- Entrez Gene ID 3691
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 439-9B monoclonal antibody specifically recognizes CD104, integrin β4 chain, a 205 kDa transmembrane glycoprotein, which associates with CD49f (integrin α6 chain) to form the α6/β4 (CD49f/CD104) complex. It is expressed on epithelial cells, Schwann cells, and some tumor cells. The CD49f/CD104 complex is located in the hemidesmosomes of the epidermis, suggesting its role in epidermal cell-basement membrane adhesion. The clone 439-9B was clustered as CD104 at the fifth Human Leucocyte Differentiation Antigen International Workshop. It may be used for immunoprecipitation, immunoblotting and immunohistochemistry on frozen tissue sections.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.