PE Mouse Anti-Human CD44v6
Clone 2F10 (RUO)
- Brand BD Pharmingen™
- Alternative Name CD44v6; CD44 v6; CD44 containing exon v6
- Vol. Per Test 5 µl
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing) Rhesus (Reported)
Flow cytometry (Routinely Tested)
- Immunogen Recombinant human CD44v3-10 Protein
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 2F10 monoclonal antibody specifically recognizes exon v6-containing isoforms of the CD44 adhesion receptor (CD44v6). CD44 is a widely-expressed, 85-250 kDa type I transmembrane glycoprotein that regulates cellular adhesion, growth, and migration and is involved in cell-cell interactions. CD44 is a member of the hyaladherin family of hyaluronan-binding proteins. It serves as a multifunctional receptor for hyaluronic acid and other ligands including collagens, fibronectin, matrix metalloproteinases, and osteopontin. The standard form of CD44 (CD44s) contains an extracellular region with a hyaluronan-binding link domain and a stem region devoid of variable exon sequences that are followed by a transmembrane domain and a cytoplasmic tail. The cytoplasmic region is linked to the cytoskeleton and may be involved in signaling interactions. Alternative splicing of CD44 gene transcripts results in the differential inclusion of 10 variant exons (v1-10) within the stem region, either individually or in multiple combinations. This results in a large number of variant CD44 (CD44v) isoforms with different cell surface expression levels and functions. CD44 isoforms containing exon v6 (CD44v6) are variably expressed on epithelial cells, monocytes, macrophages, dendritic cells, and Langerhans cells. It is expressed on certain tumor cells and on cancer stem cells (CSCs) and is implicated in tumor cell metastasis. In addition to playing roles in cellular adhesion and migration, CD44 isoforms with exon v6 sequences may serve as coreceptors for some growth factors and cytokines including hepatocyte growth factor (HGF) which is involved in driving c-Met signaling.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.