Multiparameter flow cytometric analysis of TREM-1(CD354) expression on human peripheral blood leucocytes. Whole blood was stained with either PE Mouse IgG1 κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human TREM-1 (CD354) antibody (Cat. No. 565555; Right Panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of TREM-1 (CD354) [or Ig Isotype control staining] versus side-light scatter (SSC) signals were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
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Multiparameter flow cytometric analysis of TREM-1(CD354) expression on human peripheral blood leucocytes. Whole blood was stained with either PE Mouse IgG1 κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human TREM-1 (CD354) antibody (Cat. No. 565555; Right Panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of TREM-1 (CD354) [or Ig Isotype control staining] versus side-light scatter (SSC) signals were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
BD Pharmingen™
TREM1, Triggering receptor expressed on myeloid cells 1
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human Trem-1 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
AB_2739293
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate CE_IVD
Size
100 mL
Cat No.
349202
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
565555 Rev. 2
Antibody Details
6B1
The 6B1monoclonal antibody specifically binds to TREM-1 (Triggering Receptor Expressed on Myeloid Cells 1 or Triggering Receptor Expressed on Monocytes 1) which is also known as CD354. CD354 is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. CD354 is highly expressed by neutrophils and monocytes. It is also reportedly expressed at lower levels by a variety of cells including subsets of T and B cells, NK cells and dendritic cells. Its expression is upregulated on leucocytes in response to microbial lipopolysaccharide, eg, during microbial infections or sepsis. CD354 activation triggers the release of proinflammatory cytokines and chemokines. A soluble form of CD354 has been described that may play a protective role in some infectious disease states.
565555 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565555 Rev.2
Citations & References
Development References (5)
-
Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000; 164(10):4991-4995. (Biology). View Reference
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Ford JW, McVicar DW. TREM and TREM-like receptors in inflammation and disease. Curr Opin Immunol. 2009; 21(1):38-46. (Biology). View Reference
-
Haselmayer P, Grosse-Hovest L, von Landenberg P, Schild H, Radsak MP. TREM-1 ligand expression on platelets enhances neutrophil activation. Blood. 2007; 110(3):1029-1035. (Immunogen: Functional assay, Inhibition, Neutralization). View Reference
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Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P. New B-cell CD molecules. Immunol Lett. 2011; 134(2):104-112. (Biology). View Reference
-
Radsak MP, Taube C, Haselmayer P, et al. Soluble triggering receptor expressed on myeloid cells 1 is released in patients with stable chronic obstructive pulmonary disease. Clin Dev Immunol. 2007; 52040:1-7. (Immunogen: ELISA, Flow cytometry). View Reference
565555 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
