Two-parameter flow cytometric analysis of CD263 (TRAIL-R3) expression on human peripheral blood leucocyte populations. Whole blood cells were stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557714, Left Panel) or Alexa Fluor® 647 Mouse anti-Human CD263 antibody (Cat. No. 565458; Right Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-parameter flow cytometric contour plots showing the correlated expression of CD263 (TRAIL-R3) [or Ig Isotype control staining] versus Side Light-scatter (SSC) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System.
Two-parameter flow cytometric analysis of CD263 (TRAIL-R3) expression on human peripheral blood leucocyte populations. Whole blood cells were stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557714, Left Panel) or Alexa Fluor® 647 Mouse anti-Human CD263 antibody (Cat. No. 565458; Right Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-parameter flow cytometric contour plots showing the correlated expression of CD263 (TRAIL-R3) [or Ig Isotype control staining] versus Side Light-scatter (SSC) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System.
Product Details
BD Pharmingen™
TRAIL-R3; TRAILR3; TRAIL Receptor 3; TNFRSF10C ; TR10C; DCR1; LIT; TRID
Human (QC Testing)
Mouse BALB/c IgG1
Recombinant human TRAIL R3/Fc Protein
Flow cytometry (Routinely Tested)
5 µl/test
HLDA8
AB_2739246
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate CE_IVD
Size
100 mL
Cat No.
349202
Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO
Size
100 Tests
Cat No.
557714
565458 Rev. 1
Antibody Details
B-D44
The B-D44 monoclonal antibody specifically recognizes CD263 which is also known as, TNF-related apoptosis-inducing ligand receptor 3 (TRAIL Receptor 3/TRAIL-R3), Decoy receptor 1 (DCR1), Lymphocyte inhibitor of TRAIL (LIT), or TRID. CD263 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that belongs to the TNF Receptor Superfamily which includes other TRAIL (CD253/APO-2 Ligand) Receptors: CD261 (TRAIL-R1), CD262 (TRAIL-R2), and CD264 (TRAIL-R4). Unlike CD261 or CD262 that contain an intracellular signaling death domain, CD263 (and CD264) lacks a cytoplasmic death domain and is unable to transduce apoptotic signals upon binding TRAIL. CD263 thus serves as a decoy receptor for TRAIL. It functions as a negative regulator of apoptotic signaling by competing with CD261 and CD262 for the binding of TRAIL. Overexpression of CD263 can reportedly attenuate TRAIL-induced apoptosis.
565458 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
565458 Rev.1
Citations & References
Development References (6)
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Baritaki S, Katsman A, Chatterjee D, Yeung KC, Spandidos DA, Bonavida B. Regulation of tumor cell sensitivity to TRAIL-induced apoptosis by the metastatic suppressor Raf kinase inhibitor protein via Yin Yang 1 inhibition and death receptor 5 up-regulation. J Immunol. 2007; 179(8):5441-5453. (Biology). View Reference
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Bisgin A, Terzioglu E, Aydin C, et al. TRAIL death receptor-4, decoy receptor-1 and decoy receptor-2 expression on CD8+ T cells correlate with the disease severity in patients with rheumatoid arthritis. BMC Musculoskelet Disord. 2010; 11(192):200. (Biology). View Reference
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Ch'en PF, Xu XG, Liu XS, et al. Characterisation of monoclonal antibodies to the TNF and TNF receptor families. Cell Immunol. 2005; 236(1-2):78-85. (Biology). View Reference
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Gottwald L, Piekarski J, Kubiak R, et al. Membrane expression of TRAIL receptors DR4, DR5, DcR1 and DcR2 in the normal endometrium, atypical endometrial hyperplasia and endometrioid adenocarcinoma: a tissue microarray study. Arch Gynecol Obstet. 2013; 4(889):899. (Biology). View Reference
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Mérino D, Lalaoui N, Morizot A, Schneider P, Solary E, Micheau O. Differential inhibition of TRAIL-mediated DR5-DISC formation by decoy receptors 1 and 2. Mol Cell Biochem. 2006; 26(19):7046-7055. (Biology). View Reference
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Vermot-Desroches C, Sergent E, Bonnin B, Wijdenes J. Characterization of monoclonal antibodies directed against trail or trail receptors. Cell Immunol. 2005; 236(1-2):86-91. (Clone-specific: Flow cytometry). View Reference
565458 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
